Afifi M S, Kumar V, Bennett M
J Immunol. 1985 Jun;134(6):3739-45.
Lethally irradiated mice were infused with syngeneic, H-2 allogeneic, parental strain, or H-2 heterozygous bone marrow cells. They were injected daily with rabbit anti-mouse interferons (IFN)-alpha/beta or gamma or with IFN-alpha/beta. The growth of donor-derived cells was judged 5 days later by measuring splenic incorporation of 5-iodo-2'-deoxyuridine-125I into DNA. Antibodies to IFN-alpha/beta, but not to IFN-gamma, weakened genetic (both hybrid and allogeneic) resistance to marrow cell grafts. IFN-alpha/beta stimulated hybrid and allogeneic resistance, the latter even in genetically "poor responder" mice. Mice pretreated with silica, which weakens genetic resistance, were stimulated by IFN-alpha/beta to resist incompatible marrow cell grafts; however, IFN-alpha/beta failed to reverse the effects of antiasialo GM1 serum on marrow graft rejection. IFN-alpha/beta did not inhibit the growth of syngeneic marrow cells and did not stimulate resistance to H-2 heterozygous bone marrow cells. We propose that genetic resistance occurs in two discrete steps. In the first step, hemopoietic histocompatibility (Hh) antigens are recognized by one host cell type, and this recognition leads to IFN-alpha/beta secretion by a silica-sensitive cell. In the second step, asialo GM1-positive natural killer cells stimulated by IFN-alpha/beta recognize Hh antigens on marrow stem cells and cause rejection. The defects in resistance observed in genetically poor responder mice and in mice treated with silica appear to involve the first step in recognition. The lack of rejection of H-2 heterozygous (Hh-) marrow cells by parental strain mice injected with IFN-alpha/beta indicated that specific Hh recognition is critical in the second step of genetic resistance.
对受到致死剂量照射的小鼠输注同基因、H-2 异基因、亲代品系或 H-2 杂合骨髓细胞。每天给它们注射兔抗小鼠干扰素（IFN）-α/β 或 γ 或注射 IFN-α/β。5 天后，通过测量脾脏中 5-碘-2'-脱氧尿苷-125I 掺入 DNA 的情况来判断供体来源细胞的生长。抗 IFN-α/β 抗体而非抗 IFN-γ 抗体削弱了对骨髓细胞移植的遗传（杂种和异基因）抗性。IFN-α/β 刺激杂种和异基因抗性，后者甚至在遗传上“低反应性”小鼠中也能出现。用二氧化硅预处理的小鼠，其遗传抗性减弱，但 IFN-α/β 能刺激它们抵抗不相容的骨髓细胞移植；然而，IFN-α/β 未能逆转抗唾液酸 GM1 血清对骨髓移植排斥的影响。IFN-α/β 不抑制同基因骨髓细胞的生长，也不刺激对 H-2 杂合骨髓细胞的抗性。我们提出遗传抗性分两个离散步骤发生。第一步，一种宿主细胞类型识别造血组织相容性（Hh）抗原，这种识别导致二氧化硅敏感细胞分泌 IFN-α/β。第二步，受 IFN-α/β 刺激的唾液酸 GM1 阳性自然杀伤细胞识别骨髓干细胞上的 Hh 抗原并导致排斥。在遗传上低反应性小鼠和用二氧化硅处理的小鼠中观察到的抗性缺陷似乎涉及识别的第一步。注射 IFN-α/β 的亲代品系小鼠对 H-2 杂合（Hh-）骨髓细胞缺乏排斥表明，特异性 Hh 识别在遗传抗性的第二步中至关重要。
