Hami Javad, Vafaei-Nezhad Saeed, Haghir Delaram, Haghir Hossein
Department of Anatomical Sciences, School of Medicine, Birjand University of Medical Sciences, Birjand, Iran.
School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
J Mol Neurosci. 2016 Feb;58(2):221-32. doi: 10.1007/s12031-015-0661-z. Epub 2015 Oct 13.
Insulin-like growth factor-1 (IGF-1) has an important role in development of the central nervous system (CNS). Maternal diabetes is associated with a higher risk of developmental abnormalities in their offspring including motor dysfunction and learning deficits. The present study aimed to investigate the effects of maternal diabetes on the distribution pattern of IGF-1 receptor (IGF-1R) in the developing rat cerebellar cortex. Wistar female rats were maintained diabetic from a week before pregnancy through parturition, and male offspring was killed at P0, P7, and P14. In spite of P0, there was a significant increase in the total cerebellar volume in the pups born to diabetic mothers. In diabetic group, the IGF-1R+ granular cell densities in internal granular (IGL) and molecular (ML) layers were increased at P0. Moreover, the number of positive granular and Purkinje cells in the IGL of diabetic neonates' cerebellum was reduced in comparison with the control group at P7 and P14. There were no differences either in volume or in the number of IGF-1R+ cells in the layers of the cerebellar cortex between the insulin-treated diabetic group and controls. Our data indicate that diabetes in pregnancy strikingly influence the localization of IGF-1R in the developing cerebellar cortex.
胰岛素样生长因子-1（IGF-1）在中枢神经系统（CNS）发育中起重要作用。母体糖尿病与其后代发育异常的较高风险相关，包括运动功能障碍和学习缺陷。本研究旨在调查母体糖尿病对发育中大鼠小脑皮质中IGF-1受体（IGF-1R）分布模式的影响。Wistar雌性大鼠从怀孕前一周至分娩期间维持糖尿病状态，雄性后代在出生后第0天（P0）、第7天（P7）和第14天（P14）处死。尽管在P0时没有差异，但糖尿病母亲所生幼崽的小脑总体积显著增加。在糖尿病组中，P0时内颗粒层（IGL）和分子层（ML）中IGF-1R +颗粒细胞密度增加。此外，与对照组相比，糖尿病新生儿小脑IGL中阳性颗粒细胞和浦肯野细胞的数量在P7和P14时减少。胰岛素治疗的糖尿病组和对照组之间，小脑皮质各层中IGF-1R +细胞的体积或数量均无差异。我们的数据表明，孕期糖尿病显著影响发育中小脑皮质中IGF-1R的定位。
