Yin Jun-Jie, Liang Bo, Zhan Xin-Rong
Department of Hematology, Xinxiang Central Hospital Xinxiang 453000, Henan Province, China.
Int J Clin Exp Pathol. 2015 Aug 1;8(8):9189-95. eCollection 2015.
MicroRNAs (miRNAs) are a group of small non-coding RNAs that play important roles in the pathogenesis of human diseases by negatively regulating gene expression. The aim of this study was to explore the effect of miR-204 on cell proliferation migration and invasion in T-cell acute lymphoblastic leukaemia (T-ALL).
miR-204 expression was determined in bone marrow samples from 32 leukemia patients and 32 healthy controls by quantitative real-time PCR (qRT-PCR). The effect of miR-204 on cell proliferation was evaluated by CCK8 assay, cell migration and invasion were evaluated by transwell migration and invasion assays, In addition, the regulation of SOX4 by miR-204 was evaluated by luciferase reporter assay and western blot.
our results revealed that miR-204 was low expressed in T-ALL. Cell proliferation assay showed that the cell proliferation ability was inhibited by miR-204 mimics. Moreover, migration and invasion assay suggested that overexpression of miR-204 could significantly suppressed the migration and invasion ability of T-ALL cells. Luciferase reporter assay confirmed that miR-204 directly bound to the 3' untranslated region of SOX4, and western blot suggested that miR-204 inhibited the expression of SOX4 at the protein levels.
Our findings indicated that miR-204 negatively regulates SOX4 and inhibited proliferation, migration and invasion of T-ALL cell lines. Thus, miR-204 might represent a potential therapeutic target for T-ALL intervention.
微小RNA（miRNA）是一类小的非编码RNA，通过负向调节基因表达在人类疾病的发病机制中发挥重要作用。本研究旨在探讨miR-204对T细胞急性淋巴细胞白血病（T-ALL）细胞增殖、迁移和侵袭的影响。
采用定量实时PCR（qRT-PCR）检测32例白血病患者和32例健康对照者骨髓样本中miR-204的表达。通过CCK8法评估miR-204对细胞增殖的影响，通过Transwell迁移和侵袭实验评估细胞迁移和侵袭能力。此外，通过荧光素酶报告基因实验和蛋白质印迹法评估miR-204对SOX4的调控作用。
我们的结果显示，miR-204在T-ALL中低表达。细胞增殖实验表明，miR-204模拟物可抑制细胞增殖能力。此外，迁移和侵袭实验表明，miR-204过表达可显著抑制T-ALL细胞的迁移和侵袭能力。荧光素酶报告基因实验证实miR-204直接与SOX4的3'非翻译区结合，蛋白质印迹法表明miR-204在蛋白质水平上抑制SOX4的表达。
我们的研究结果表明，miR-204负向调节SOX4，并抑制T-ALL细胞系的增殖、迁移和侵袭。因此，miR-204可能是T-ALL干预的潜在治疗靶点。
