Wang Nai-Guo, Wang Da-Chuan, Tan Bing-Yi, Wang Feng, Yuan Ze-Nong
Department of Spinal Surgery, Shandong Provincial Hospital Affiliated to Shandong University Jinan 250021, Shandong, China.
Int J Clin Exp Pathol. 2015 Aug 1;8(8):9314-9. eCollection 2015.
Potential values of microRNA152 (miR-152) as a serum diagnostic and prognostic biomarker have not been determined in human osteosarcoma. By detecting the expression of miR-152 among 80 osteosarcoma patients, 20 periostitis patients and 20 healthy individuals using qRT-PCR, we aimed to explore the clinical significance of miR-152 in osteosarcoma patients. The expression of miR-152 was significantly decreased in patients with osteosarcoma compared to patients with periostitis (P<0.01) and healthy controls (P<0.01). The relationship between clinicopathologic characteristics and miR-152 was analyzed by chi-square test. The outcome indicated that miR-152 might be linked with the development of osteosarcoma. Moreover, the receiver operating characteristic (ROC) curve was performed to estimate the diagnostic value of miR-152. The result demonstrated that miR-152 might be a promising diagnostic marker of osteosarcoma with an AUC of 0.956, combing with 92.5% specificity and 96.2% sensitivity. The relationship between miR-152 and overall survival of osteosarcoma patients was analyzed by Kaplan-Meier curve and log rank test. As a result, the survival time of patients with low miR-152 expression was significantly shorter than those with high miR-152 expression (P<0.001). Then Cox regression analysis was used to estimate the prognostic value of miR-152 in osteosarcoma. The outcomes showed that low miR-152 expression (P=0.004) might be a potential independent prognostic marker for osteosarcoma patients. These findings suggested that down-regulation of miR-152 could be considered as a predictor for diagnosis and prognosis of osteosarcoma patients.
微小RNA152（miR - 152）作为血清诊断和预后生物标志物在人类骨肉瘤中的潜在价值尚未确定。通过使用qRT - PCR检测80例骨肉瘤患者、20例骨膜炎患者和20例健康个体中miR - 152的表达，我们旨在探讨miR - 152在骨肉瘤患者中的临床意义。与骨膜炎患者（P<0.01）和健康对照（P<0.01）相比，骨肉瘤患者中miR - 152的表达显著降低。采用卡方检验分析临床病理特征与miR - 152之间的关系。结果表明，miR - 152可能与骨肉瘤的发生发展有关。此外，绘制受试者工作特征（ROC）曲线以评估miR - 152的诊断价值。结果表明，miR - 152可能是一种有前景的骨肉瘤诊断标志物，曲线下面积（AUC）为0.956，特异性为92.5%，敏感性为96.2%。采用Kaplan - Meier曲线和对数秩检验分析miR - 152与骨肉瘤患者总生存期的关系。结果显示，miR - 152表达低的患者生存时间明显短于miR - 152表达高的患者（P<0.001）。然后使用Cox回归分析评估miR - 152在骨肉瘤中的预后价值。结果显示，miR - 152低表达（P = 0.004）可能是骨肉瘤患者潜在的独立预后标志物。这些发现表明，miR - 152的下调可被视为骨肉瘤患者诊断和预后的预测指标。
