通过导入含有两个Bt杀虫基因和一个EPSPS基因的T-DNA来培育抗虫耐草甘膦水稻。
Generation of insect-resistant and glyphosate-tolerant rice by introduction of a T-DNA containing two Bt insecticidal genes and an EPSPS gene.
作者信息
Zhao Qi-chao, Liu Ming-hong, Zhang Xian-wen, Lin Chao-yang, Zhang Qing, Shen Zhi-cheng
机构信息
State Key Laboratory of Rice Biology, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China.
出版信息
J Zhejiang Univ Sci B. 2015 Oct;16(10):824-31. doi: 10.1631/jzus.B1500056.
Insect resistance and glyphosate tolerance have been two of the most important traits in the genetic improvement of various crops. In this study, two Bacillus thuringiensis (Bt) insecticidal genes, Cry1Ac and Cry1Ig, and a modified glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene (G10) were combined into a single transferred DNA (T-DNA) fragment and introduced into rice by Agrobacterium-mediated transformation. A transgenic line with single-copy T-DNA insertion named GAI-14 was found to be highly resistant to striped stem borer and rice leaf roller, and tolerant to glyphosate. Analysis of T-DNA border sequence suggested that the transgenes were inserted at the chromosome 3 and appeared to have not interrupted any known or putative genes. A field trial observed no significant difference in the basic agronomic traits between GAI-14 and the recipient rice.
抗虫性和耐草甘膦性一直是各种作物遗传改良中最重要的两个性状。在本研究中，将两个苏云金芽孢杆菌（Bt）杀虫基因Cry1Ac和Cry1Ig，以及一个经过修饰的耐草甘膦5-烯醇丙酮酸莽草酸-3-磷酸合酶（EPSPS）基因（G10）组合成一个单一的转移DNA（T-DNA）片段，并通过农杆菌介导的转化方法导入水稻。发现一个单拷贝T-DNA插入的转基因株系GAI-14对二化螟和稻纵卷叶螟具有高度抗性，并且对草甘膦具有耐受性。T-DNA边界序列分析表明，转基因插入到3号染色体上，且似乎没有中断任何已知或推测的基因。田间试验观察到GAI-14与受体水稻之间的基本农艺性状没有显著差异。
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