Stacking Multiple Genes Improves Resistance to , , and in Transgenic Rice.

The ability of various pests and diseases to adapt to a single plant resistance gene over time leads to loss of resistance in transgenic rice. Therefore, introduction of different pest and disease resistance genes is critical for successful cultivation of transgenic rice strains with broad-spectrum resistance to multiple pathogens. Here, we produced resistance rice lines with multiple, stacked resistance genes by stacking breeding and comprehensively evaluated their resistance to (striped rice stemborer), (rice blast), and (brown planthopper) in a pesticide-free environment. and are exogenous genes from . , and are natural genes in rice. CH121TJH was introduced into , , and . CH891TJH and R205XTJH were introduced into , , and . Compared with those observed in their recurrent parents, CH121TJH significantly increased the mortality of borers. The other two lines CH891TJH and R205XTJH are the same result. Three lines introduction of and significantly reduced the area of rice blast lesions, and introduction of significantly reduced seedling mortality from . Introduction of the exogenous genes had relatively few effects on agronomic and yield traits of the original parents. These findings suggest that stacking of rice resistance genes through molecular marker-assisted backcross breeding can confer broad spectrum and multiple resistance in differently genetic backgrounds.