Kong Baohua, Niu Haili, Sun Fangda, Han Jianchun, Liu Qian
College of Food Science, Northeast Agricultural University, Harbin, Heilongjiang 150030, China; Synergetic Innovation Center of Food Safety and Nutrition, Harbin, Heilongjiang 150030, China.
College of Food Science, Northeast Agricultural University, Harbin, Heilongjiang 150030, China.
Int J Biol Macromol. 2016 Jan;82:637-44. doi: 10.1016/j.ijbiomac.2015.10.026. Epub 2015 Oct 23.
The objective of this study was to investigate the regulatory effect of porcine plasma protein hydrolysates (PPPH) on the physicochemical, pasting, and gelatinization properties of corn starch (CS). The results showed that the solubility of CS markedly increased, whereas swelling power and gel penetration force decreased with increased PPPH concentration (P<0.05). Compared with native CS, PPPH significantly lowered peak viscosity, minimum viscosity, final viscosity, and total setback, whereas it increased breakdown and pasting temperature in rapid visco analyzer (RVA) measurement (P<0.05) and obviously enhanced the gelatinization temperature as determined in differential scanning calorimetry (DSC) (P<0.05). Confocal laser scanning microscopy (CLSM) showed that PPPH surrounded the starch granules at room temperature (25°C) and then formed a network with swollen starch granules during gelatinization. Atomic force microscopy (AFM) images indicated that the blocklet sizes of gelatinized CS-PPPH mixtures were smaller and more uniform than native CS. The results proved that pasting and gelatinization abilities of CS can be effectively influenced by adding PPPH.
本研究的目的是探究猪血浆蛋白水解物(PPPH)对玉米淀粉(CS)的物理化学、糊化及凝胶化特性的调节作用。结果表明,随着PPPH浓度的增加,CS的溶解度显著增加,而膨胀力和凝胶穿透强度降低(P<0.05)。与天然CS相比,在快速粘度分析仪(RVA)测量中,PPPH显著降低了峰值粘度、最低粘度、最终粘度和总回生值,而增加了破损值和糊化温度(P<0.05),并且在差示扫描量热法(DSC)测定中明显提高了凝胶化温度(P<0.05)。共聚焦激光扫描显微镜(CLSM)显示,PPPH在室温(25°C)下包围淀粉颗粒,然后在凝胶化过程中与膨胀的淀粉颗粒形成网络。原子力显微镜(AFM)图像表明,糊化的CS-PPPH混合物的块状尺寸比天然CS更小且更均匀。结果证明,添加PPPH可有效影响CS的糊化和凝胶化能力。