一种针对组蛋白去乙酰化酶1前期磷酸化的单克隆抗体：早期有丝分裂细胞的一种检测指标
A monoclonal antibody specific for prophase phosphorylation of histone deacetylase 1: a readout for early mitotic cells.
作者信息
Segré Chiara V, Senese Silvia, Loponte Sara, Santaguida Stefano, Soffientini Paolo, Grigorean Gabriela, Cinquanta Mario, Ossolengo Giuseppe, Seiser Christian, Chiocca Susanna
机构信息
a Department of Experimental Oncology ; European Institute of Oncology ; Via Adamello 16; 20139 Milan , Italy.
b Present addresses: Fondazione Umberto Veronesi ; Piazza Velasca 5; 20121 Milano , Italy.
出版信息
MAbs. 2016;8(1):37-42. doi: 10.1080/19420862.2015.1098795. Epub 2015 Oct 14.
Abstract
Histone deacetylases (HDACs) are modification enzymes that regulate a plethora of biological processes. HDAC1, a crucial epigenetic modifier, is deregulated in cancer and subjected to a variety of post-translational modifications. Here, we describe the generation of a new monoclonal antibody that specifically recognizes a novel highly dynamic prophase phosphorylation of serine 406-HDAC1, providing a powerful tool for detecting early mitotic cells.
摘要
组蛋白脱乙酰酶（HDACs）是调节众多生物学过程的修饰酶。HDAC1是一种关键的表观遗传修饰因子，在癌症中失调，并经历多种翻译后修饰。在此，我们描述了一种新的单克隆抗体的产生，该抗体特异性识别丝氨酸406-HDAC1的一种新型高度动态的前期磷酸化，为检测早期有丝分裂细胞提供了一种强大的工具。
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