被抗体捕获的甲型流感病毒基质蛋白2细胞外结构域保守氨基末端的晶体结构
Crystal Structure of the Conserved Amino Terminus of the Extracellular Domain of Matrix Protein 2 of Influenza A Virus Gripped by an Antibody.
作者信息
Cho Ki Joon, Schepens Bert, Moonens Kristof, Deng Lei, Fiers Walter, Remaut Han, Saelens Xavier
机构信息
Medical Biotechnology Center, VIB, Ghent, Belgium Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium.
Structural and Molecular Microbiology, Structural Biology Research Center, VIB, Brussels, Belgium Structural Biology Brussels, Vrije Universiteit Brussel, Brussels, Belgium.
出版信息
J Virol. 2015 Oct 14;90(1):611-5. doi: 10.1128/JVI.02105-15. Print 2016 Jan 1.
Abstract
We report the crystal structure of the M2 ectodomain (M2e) in complex with a monoclonal antibody that binds the amino terminus of M2. M2e extends into the antibody binding site to form an N-terminal β-turn near the bottom of the paratope. This M2e folding differs significantly from that of M2e in complex with an antibody that binds another part of M2e. This suggests that M2e can adopt at least two conformations that can elicit protective antibodies.
摘要
我们报道了M2胞外结构域（M2e）与一种结合M2氨基末端的单克隆抗体形成复合物的晶体结构。M2e延伸至抗体结合位点，在互补决定区底部附近形成一个N端β转角。这种M2e折叠与M2e和一种结合M2e另一部分的抗体形成复合物时的折叠有显著差异。这表明M2e至少可以采用两种能引发保护性抗体的构象。
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