Jais Murni H, Md Zin Reena R, Muhd Hanapi Nur A, Md Ali Siti A
Departments of *Pathology, Medical Faculty †Diagnostic Laboratory Medicine, UKM Medical Centre, Kuala Lumpur ‡Medical Laboratory Science Cluster, Faculty of Medicine, UiTM, Selangor, Malaysia.
Appl Immunohistochem Mol Morphol. 2017 Jan;25(1):44-48. doi: 10.1097/PAI.0000000000000258.
Ezrin, a membrane-linking protein, has been shown to play an important role in the carcinogenesis of infiltrating breast ductal carcinoma and its strong expression has been used to predict poor prognosis in patients with breast carcinoma. In this study, we compared ezrin protein distribution pattern in benign breast disease and breast cancer molecular subtypes and evaluated their association with clinicopathologic variables. A total of 376 breast cases (142 benign and 234 malignant cases) were studied. Immunohistochemical analysis for ezrin was performed and its expression was observed in terms of its distribution, intensity, and proportion of cells reactive for ezrin. Ezrin was expressed in all benign cases and 91.7% of malignant cases. Apical staining was positively associated with benign breast disease, whereas membranous and cytoplasmic staining were more frequently observed in malignant cases, specifically of hormone receptor-positive subtypes (luminal A and luminal B). Ezrin was significantly overexpressed in luminal A, luminal B, and HER2 subtypes. Reduced ezrin expression was significantly associated with triple-negative breast cancer molecular subtype. No significant association was demonstrated between ezrin expression and Her2 gene amplification, tumor grading, or staging.
埃兹蛋白是一种膜连接蛋白，已被证明在浸润性乳腺导管癌的致癌过程中起重要作用，其高表达已被用于预测乳腺癌患者的不良预后。在本研究中，我们比较了埃兹蛋白在乳腺良性疾病和乳腺癌分子亚型中的蛋白分布模式，并评估了它们与临床病理变量的相关性。共研究了376例乳腺病例（142例良性病例和234例恶性病例）。对埃兹蛋白进行免疫组织化学分析，并从其分布、强度和埃兹蛋白反应性细胞比例方面观察其表达情况。埃兹蛋白在所有良性病例和91.7%的恶性病例中均有表达。顶端染色与乳腺良性疾病呈正相关，而膜性和胞质染色在恶性病例中更常见，特别是激素受体阳性亚型（管腔A型和管腔B型）。埃兹蛋白在管腔A型、管腔B型和HER2亚型中显著过表达。埃兹蛋白表达降低与三阴性乳腺癌分子亚型显著相关。埃兹蛋白表达与Her2基因扩增、肿瘤分级或分期之间未显示出显著相关性。
