Lin Ching-Ping, Lo Hsiao-Feng, Chen Chien-Yu, Chen Long-Fang Oliver
a Institute of Plant and Microbial Biology, Academia Sinica , Nankang , Taipei , Taiwan .
b Department of Earth and Life Science , University of Taipei , Taipei , Taiwan .
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Sep;27(5):3731-2. doi: 10.3109/19401736.2015.1079879. Epub 2015 Oct 15.
The entire mitogenome of the Vigna radiata var. radiata NM92 was identified as a circular molecule of 401 262 bp length (DDBJ accession number: AP014716). The contents of A, T, C, and G in the NM92 mitogenome were found to be 27.48%, 27.41%, 22.63%, and 22.48%, respectively. The NM92 mitogenome encoded 3 rRNAs, 16 tRNAs and 33 proteins. Eight protein-coding genes (nad1, nad2, nad4, nad5, nad7, rps3, and rps10) centain introns. Among them, three (nad1, nad2, and nad5) are trans-spliced genes. A phylogenetic tree was reconstructed using the 21 protein-coding genes of 16 crops. A species of gymnosperms, Cycas, was selected as the outgroup. This complete mitogenome sequence provides useful information to understand the cultivation of Vigna radiata and other crops.
绿豆变种NM92的完整线粒体基因组被鉴定为一个长度为401262 bp的环状分子（DDBJ登录号：AP014716）。发现NM92线粒体基因组中A、T、C和G的含量分别为27.48%、27.41%、22.63%和22.48%。NM92线粒体基因组编码3种rRNA、16种tRNA和33种蛋白质。8个蛋白质编码基因（nad1、nad2、nad4、nad5、nad7、rps3和rps10）含有内含子。其中，3个（nad1、nad2和nad5）是反式剪接基因。使用16种作物的21个蛋白质编码基因重建了系统发育树。选择一种裸子植物苏铁作为外类群。这个完整的线粒体基因组序列为了解绿豆和其他作物的栽培提供了有用的信息。
