Yan Dan-Kan, Hu Min, Tang Yun-Xia, Fan Jia-Qin
College of Plant Protection, Nanjing Agricultural University and Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing 210095, China. College of Plant Protection, Nanjing Agricultural University and Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing 210095, China. Institute of Plant Protection and Agro-Products Safety, Anhui Academy of Agricultural Sciences, Hefei 230031, China.
College of Plant Protection, Nanjing Agricultural University and Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing 210095, China. Institute of Plant Protection and Agro-Products Safety, Anhui Academy of Agricultural Sciences, Hefei 230031, China.
J Econ Entomol. 2015 Aug;108(4):2000-8. doi: 10.1093/jee/tov139. Epub 2015 May 29.
The western flower thrips is an economically important worldwide pest of many crops, and chlorpyrifos has been used to control western flower thrips for many years. To develop a better resistance-management strategy, a chlorpyrifos-resistant strain of western flower thrips (WFT-chl) was selected in the laboratory. More than 39-fold resistance was achieved after selected by chlorpyrifos for 19 generations in comparison with the susceptible strain (WFT-S). Proteome of western flower thrips (WFT-S and WFT-chl) was investigated using a quantitative proteomics approach with isobaric tag for relative and absolute quantification technique and liquid chromatography-tandem mass spectrometry technologies. According to the functional analysis, 773 proteins identified were grouped into 10 categories of molecular functions and 706 proteins were presented in 213 kinds of pathways. Comparing the proteome of WFT-chl with that of WFT-S, a total of eight proteins were found up-regulated and three down-regulated. The results from functional annotation and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses indicated that the differentially expressed protein functions in binding, catalyzing, transporting, and enzyme regulation were most important in resistance development. A list of proteins functioning in biological processes of metabolism, biological regulation, and response to stimulus was found in WFT-chl, suggesting that they are possibly the major components of the resistance mechanism to chlorpyrifos in western flower thrips. Notably, several novel potential resistance-related proteins were identified such as ribosomal protein, Vg (vitellogenin), and MACT (muscle actin), which can be used to improve our understanding of the resistance mechanisms in western flower thrips. This study provided the first comprehensive view of the complicated resistance mechanism employed by WFT-S and WFT-chl through the isobaric tag for relative and absolute quantification coupled with liquid chromatography-tandem mass spectrometry technologies.
西花蓟马是一种在全球范围内对多种作物具有重要经济影响的害虫，多年来一直使用毒死蜱来防治西花蓟马。为了制定更好的抗性管理策略，在实验室中筛选出了一株对毒死蜱具有抗性的西花蓟马品系（WFT-chl）。与敏感品系（WFT-S）相比，经毒死蜱筛选19代后，获得了超过39倍的抗性。采用相对和绝对定量的等压标签定量蛋白质组学方法及液相色谱-串联质谱技术，对西花蓟马（WFT-S和WFT-chl）的蛋白质组进行了研究。根据功能分析，鉴定出的773种蛋白质被分为10类分子功能，706种蛋白质存在于213种途径中。比较WFT-chl和WFT-S的蛋白质组，共发现8种蛋白质上调，3种蛋白质下调。功能注释和京都基因与基因组百科全书途径富集分析结果表明，差异表达蛋白在结合、催化、转运和酶调节等功能中对抗性发展最为重要。在WFT-chl中发现了一系列在代谢、生物调节和刺激反应等生物学过程中发挥作用的蛋白质，表明它们可能是西花蓟马对毒死蜱抗性机制的主要组成部分。值得注意的是，鉴定出了几种新的潜在抗性相关蛋白，如核糖体蛋白、卵黄原蛋白（Vg）和肌肉肌动蛋白（MACT），这有助于提高我们对西花蓟马抗性机制的理解。本研究通过相对和绝对定量的等压标签结合液相色谱-串联质谱技术，首次全面揭示了WFT-S和WFT-chl所采用的复杂抗性机制。
