Ramm Crystal, Wayadande Astri, Baird Lisa, Nandakumar Renu, Madayiputhiya Nandakumar, Amundsen Keenan, Donze-Reiner Teresa, Baxendale Frederick, Sarath Gautam, Heng-Moss Tiffany
Department of Entomology, University of Nebraska, Lincoln, NE 68583.
Department of Entomology and Plant Pathology, Oklahoma State University, Stillwater, OK 74078.
J Econ Entomol. 2015 Aug;108(4):2055-64. doi: 10.1093/jee/tov149. Epub 2015 Jun 10.
The western chinch bug, Blissus occiduus Barber, is a serious pest of buffalograss, Buchloe dactyloides (Nuttall) due to physical and chemical damage caused during the feeding process. Although previous work has investigated the feeding behaviors of chinch bugs in the Blissus complex, no study to date has explored salivary gland morphology and the associated salivary complex of this insect. Whole and sectioned B. occiduus salivary glands were visualized using light and scanning electron microscopy to determine overall structure and cell types of the salivary glands and their individual lobes. Microscopy revealed a pair of trilobed principal glands and a pair of tubular accessory glands of differing cellular types. To link structure with function, the salivary gland proteome was characterized using liquid chromatography tandem mass spectrometry. The salivary proteome analysis resulted in B. occiduus sequences matching 228 nonhomologous protein sequences of the pea aphid, Acyrthosiphon pisum (Harris), with many specific to the proteins present in the salivary proteome of A. pisum. A number of sequences were assigned the molecular function of hydrolase and oxido-reductase activity, with one specific protein sequence revealing a peroxidase-like function. This is the first study to characterize the salivary proteome of B. occiduus and the first of any species in the family Blissidae.
西部麦长蝽（Blissus occiduus Barber）是野牛草（Buchloe dactyloides (Nuttall)）的一种严重害虫，因为其在取食过程中会造成物理和化学损伤。尽管之前的研究已经调查了麦长蝽属昆虫的取食行为，但迄今为止尚无研究探索该昆虫的唾液腺形态及其相关的唾液复合体。利用光学显微镜和扫描电子显微镜对完整的和切片的西部麦长蝽唾液腺进行观察，以确定唾液腺及其各个叶的整体结构和细胞类型。显微镜观察显示有一对三叶的主腺和一对细胞类型不同的管状副腺。为了将结构与功能联系起来，使用液相色谱串联质谱对唾液腺蛋白质组进行了表征。唾液蛋白质组分析结果显示，西部麦长蝽的序列与豌豆蚜（Acyrthosiphon pisum (Harris)）的228个非同源蛋白质序列匹配，其中许多序列是豌豆蚜唾液蛋白质组中特有的蛋白质。一些序列被赋予了水解酶和氧化还原酶活性的分子功能，其中一个特定的蛋白质序列显示出类似过氧化物酶的功能。这是首次对西部麦长蝽的唾液蛋白质组进行表征的研究，也是盲蝽科任何物种中的首次研究。
