通过DSPE-PEG-PEI对单壁碳纳米管进行非共价功能化用于小干扰RNA递送
Non-Covalently Functionalized of Single-Walled Carbon Nanotubes by DSPE-PEG-PEI for SiRNA Delivery.
作者信息
Siu King Sun, Zhang Yujuan, Zheng Xiufen, Koropatnick James, Min Wei-Ping
机构信息
Department of Pathology, Surgery, and Oncology, University of Western Ontario, Windermere Road, University Hospital C8-111, London, ON, N6A 5A5, Canada.
London Regional Cancer Program, Lawson Health Research Institute, London, ON, Canada.
出版信息
Methods Mol Biol. 2016;1364:151-63. doi: 10.1007/978-1-4939-3112-5_13.
The expression of a gene can be specifically downregulated by small interfering RNA (SiRNA). Modified carbon nanotubes (CNT) can be used to protect SiRNA and facilitate its entry into cells. Regardless of that, simple and efficient functionalization of CNT is lacking. Effective SiRNA delivery can be carried out using non-covalently functionalized CNT, where non-covalent (versus covalent) functionalization is simpler and more expeditious. Non-covalently functionalized single walled carbon nanotubes (SWCNT) that include a lipopolymer are described here. Polyethylenimine (PEI) conjugated to 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)-2000] (DSPE-PEG) was generated and the products used to disperse CNT to form DSPE-PEG-PEI/CNT (DGI/C), an agent capable of facilitating SiRNA delivery to cells in vitro and organs and cells in vivo.
小干扰RNA（SiRNA）可特异性下调基因表达。修饰的碳纳米管（CNT）可用于保护SiRNA并促进其进入细胞。尽管如此，CNT仍缺乏简单有效的功能化方法。使用非共价功能化的CNT可实现有效的SiRNA递送，其中非共价（相对于共价）功能化更简单、更快捷。本文描述了包含脂质聚合物的非共价功能化单壁碳纳米管（SWCNT）。生成了与1,2-二硬脂酰-sn-甘油-3-磷酸乙醇胺-N-[氨基（聚乙二醇）-2000]（DSPE-PEG）共轭的聚乙烯亚胺（PEI），并将产物用于分散CNT以形成DSPE-PEG-PEI/CNT（DGI/C），一种能够在体外促进SiRNA递送至细胞以及在体内促进其递送至器官和细胞的试剂。
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