Khodaveisi Javad, Shabani Ali Mohammad Haji, Dadfarnia Shayessteh, Moghadam Masoud Rohani, Hormozi-Nezhad Mohammad Reza
Department of Chemistry, Faculty of Science, Yazd University, 89195-741 Yazd, Iran.
Department of Chemistry, Faculty of Science, Yazd University, 89195-741 Yazd, Iran.
Spectrochim Acta A Mol Biomol Spectrosc. 2016 Jan 15;153:709-13. doi: 10.1016/j.saa.2015.10.004. Epub 2015 Oct 9.
A highly sensitive and simple indirect spectrophotometric method has been developed for the determination of trace amounts of inorganic mercury (Hg(2+)) in aqueous media. The method is based on the inhibitory effect of Hg(2+) on the activity of horseradish peroxidase (HRP) in the oxidation of ascorbic acid by hydrogen peroxide followed by the reduction of Au(3+) to Au-NPs by unreacted ascorbic acid and the measurement of the absorbance of localized surface plasmon resonance (LSPR) peak of gold nanoparticles (at 530 nm) which is directly proportional to the concentration of Hg(2+). Under the optimum conditions, the calibration curve was linear in the concentration range of 1-220 ng mL(-1). Limits of detection (LOD) and quantification (LOQ) were 0.2 and 0.7 ng mL(-1), respectively and the relative standard deviation at 100 ng mL(-1) level of Hg(2+) was 2.6%. The method was successfully applied to the determination of mercury in different water samples.
已开发出一种高灵敏度且简单的间接分光光度法，用于测定水介质中痕量无机汞（Hg(2+)）。该方法基于Hg(2+)对辣根过氧化物酶（HRP）活性的抑制作用，此酶在过氧化氢氧化抗坏血酸的过程中发挥作用，随后未反应的抗坏血酸将Au(3+)还原为金纳米粒子（Au-NPs），并通过测量金纳米粒子的局域表面等离子体共振（LSPR）峰（在530 nm处）的吸光度来实现，该吸光度与Hg(2+)的浓度成正比。在最佳条件下，校准曲线在1 - 220 ng mL(-1)的浓度范围内呈线性。检测限（LOD）和定量限（LOQ）分别为0.2和0.7 ng mL(-1)，在100 ng mL(-1) Hg(2+)水平下的相对标准偏差为2.6%。该方法已成功应用于不同水样中汞的测定。
