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P2X7R blockade prevents NLRP3 inflammasome activation and brain injury in a rat model of intracerebral hemorrhage: involvement of peroxynitrite.

作者信息

Feng Liang, Chen Yizhao, Ding Rui, Fu Zhenghao, Yang Shuo, Deng Xinqing, Zeng Jun

机构信息

The National Key Clinical Specialty, The Engineering Technology Research Center of Education Ministry of China, Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, Guangzhou, 510282, China.

Department of Neurosurgery, Jingmen No. 1 People's Hospital, Jingmen, 448000, Hubei, China.

出版信息

J Neuroinflammation. 2015 Oct 17;12:190. doi: 10.1186/s12974-015-0409-2.

DOI:10.1186/s12974-015-0409-2
PMID:26475134
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4609067/
Abstract

BACKGROUND: The NLR family, pyrin domain-containing 3 (NLRP3) inflammasome plays a key role in intracerebral hemorrhage (ICH)-induced inflammatory injury, and the purinergic 2X7 receptor (P2X7R) is upstream of NLRP3 activation. This study aimed to investigate how P2X7R functions in ICH-induced inflammatory injury and how the receptor interacts with the NLRP3 inflammasome. METHODS: Rats were treated with P2X7R small interfering RNA (siRNA) 24 h before undergoing collagenase-induced ICH. A selective P2X7R inhibitor (blue brilliant G, BBG) or a peroxynitrite (ONOO(-)) decomposition catalyst (5,10,15,20-tetrakis(4-sulfonatophenyl)porphyrinato iron(III) [FeTPPS]) was injected 30 min after ICH. Brain water content, hemorrhagic lesion volume, and neurological deficits were evaluated, and western blot, immunofluorescence, and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) were carried out. RESULTS: Striatal P2X7R and NLRP3 inflammasomes were activated after ICH. Gene silencing of P2X7R suppressed NLRP3 inflammasome activation and interleukin (IL)-1β/IL-18 release and significantly ameliorated brain edema and neurological deficits. Additionally, enhanced NADPH oxidase 2 (NOX2, gp91(phox)) and inducible nitric oxide synthase (iNOS), as well as their cytotoxic product (ONOO(-)) were markedly attenuated by BBG treatment following ICH. This was accompanied by downregulations of the inflammasome components, IL-1β/IL-18 and myeloperoxidase (MPO, a neutrophil marker). Most importantly, inflammasome activation and IL-1β/IL-18 release were significantly inhibited by ONOO(-) decomposition with FeTPPS. CONCLUSIONS: Our findings implicate that P2X7R exacerbated inflammatory progression and brain damage in ICH rats possibly via NLRP3 inflammasome-dependent IL-1β/IL-18 release and neutrophil infiltration. ONOO(-), a potential downstream signaling molecule of P2X7R, may play a critical role in triggering NLRP3 inflammasome activation.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db8f/4609067/4042ea39257f/12974_2015_409_Fig12_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db8f/4609067/9e68733b6739/12974_2015_409_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db8f/4609067/04c06f537a06/12974_2015_409_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db8f/4609067/d9d6b1527afa/12974_2015_409_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db8f/4609067/086cf5089fa5/12974_2015_409_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db8f/4609067/0eab88bc6c36/12974_2015_409_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db8f/4609067/4e81e8acc2e5/12974_2015_409_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db8f/4609067/cd59502c044d/12974_2015_409_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db8f/4609067/19e173abb7ed/12974_2015_409_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db8f/4609067/e0a0662f448f/12974_2015_409_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db8f/4609067/e9e56de9afbc/12974_2015_409_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db8f/4609067/485ca71186a4/12974_2015_409_Fig11_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db8f/4609067/4042ea39257f/12974_2015_409_Fig12_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db8f/4609067/9e68733b6739/12974_2015_409_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db8f/4609067/04c06f537a06/12974_2015_409_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db8f/4609067/d9d6b1527afa/12974_2015_409_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db8f/4609067/086cf5089fa5/12974_2015_409_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db8f/4609067/0eab88bc6c36/12974_2015_409_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db8f/4609067/4e81e8acc2e5/12974_2015_409_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db8f/4609067/cd59502c044d/12974_2015_409_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db8f/4609067/19e173abb7ed/12974_2015_409_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db8f/4609067/e0a0662f448f/12974_2015_409_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db8f/4609067/e9e56de9afbc/12974_2015_409_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db8f/4609067/485ca71186a4/12974_2015_409_Fig11_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db8f/4609067/4042ea39257f/12974_2015_409_Fig12_HTML.jpg

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本文引用的文献

[1]
Peroxynitrite decomposition catalyst prevents matrix metalloproteinase-9 activation and neurovascular injury after hemoglobin injection into the caudate nucleus of rats.

Neuroscience. 2015-6-25

[2]
Inhibition of the NLRP3 inflammasome provides neuroprotection in rats following amygdala kindling-induced status epilepticus.

J Neuroinflammation. 2014-12-17

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Antioxid Redox Signal. 2015-5-1

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Blood-brain barrier disruption induced by hemoglobin in vivo: Involvement of up-regulation of nitric oxide synthase and peroxynitrite formation.

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Toll-like receptor 2/4 heterodimer mediates inflammatory injury in intracerebral hemorrhage.

Ann Neurol. 2014-5-28

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NLRP3 deficiency ameliorates neurovascular damage in experimental ischemic stroke.

J Cereb Blood Flow Metab. 2014-1-15

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