Teplyakov A V, Kuranova I P, Harutyunyan E H, Frömmel C, Höhne W E
Institute of Crystallography, Acad. Sci., USSR.
FEBS Lett. 1989 Feb 13;244(1):208-12. doi: 10.1016/0014-5793(89)81194-9.
The crystal structure of thermitase from Thermoactinomyces vulgaris has been determined by x-ray diffraction at 2.2 A resolution. The structure was solved by a combination of single isomorphous replacement and molecular replacement methods. The structure was refined to a conventional R factor of 0.24 using restrained least square procedures CORELS and PROLSQ. The tertiary structure of thermitase is similar to that of subtilsin BPN'. The greatest differences between these structures are related to the insertions and deletions in the sequence.
嗜热放线菌嗜热酶的晶体结构已通过X射线衍射在2.2埃分辨率下测定。该结构通过单同晶置换和分子置换方法相结合得以解析。使用约束最小二乘法程序CORELS和PROLSQ将结构精修至传统R因子为0.24。嗜热酶的三级结构与枯草杆菌蛋白酶BPN'的相似。这些结构之间最大的差异与序列中的插入和缺失有关。
