Truong Alexander D, Kho Michelle E, Brower Roy G, Feldman Dorianne R, Colantuoni Elizabeth, Needham Dale M
Pulmonary, Allergy and Critical Care Medicine, Emory University, Atlanta, GA, USA.
Department of Physical Medicine and Rehabilitation, Johns Hopkins University, Baltimore, MD, USA.
Clin Physiol Funct Imaging. 2017 May;37(3):255-262. doi: 10.1111/cpf.12290. Epub 2015 Oct 16.
The effect of exercise on cytokines may improve muscle strength. Neuromuscular electrical stimulation (NMES) is a muscle-preserving therapy that benefits patients unable to participate in active exercise. How NMES alters cytokines is unclear. The aim of this study was to study the effects of 1 NMES session on cytokines associated with protein metabolism during exercise.
We evaluated the effects of NMES on IL-1, IL-6, IL-10 and TNF-α levels in peripheral blood. Participants received NMES to bilateral lower extremity muscles (quadriceps, tibialis anterior, gastrocnemius) for 30 min. Blood samples immediately pre- and post-NMES were drawn at 15-min intervals to 2-h follow-up, and the mean values of pre-NMES levels were compared to peak and trough post-NMES levels. For cytokines with significant changes, we conducted a repeated-measures linear regression analysis. We also measured post-NMES lactate and creatine kinase levels.
We enrolled nine eligible participants. There was a significant increase in peak IL-6 from the mean pre-NMES value [0·65 (0·89) to 1·04 (0·89) pg ml , P = 0·001] and a significant decrease in trough IL-1 [0·08 (0·07) to 0·02 (0·02) pg ml , P = 0·041] and TNF-α [2·42 (0·54) to 2·16 (0·59) pg ml , P = 0·021]. In repeated-measures regression analysis, we identified significantly higher mean IL-6 values throughout the full 120 min post-NMES period, and a significantly higher mean IL-1 value at 30 min post-NMES. There were no significant differences in peak IL-10, trough IL-6, lactate, or creatine kinase values.
In nine healthy humans, 30 min of NMES was temporally associated with changes in cytokines similar to the effects of active exercise and may mediate NMES' observed effects on reducing muscle weakness.
运动对细胞因子的影响可能会增强肌肉力量。神经肌肉电刺激（NMES）是一种保护肌肉的治疗方法，对无法参与主动运动的患者有益。NMES如何改变细胞因子尚不清楚。本研究的目的是探讨单次NMES治疗对运动期间与蛋白质代谢相关的细胞因子的影响。
我们评估了NMES对外周血中白细胞介素-1（IL-1）、白细胞介素-6（IL-6）、白细胞介素-10（IL-10）和肿瘤坏死因子-α（TNF-α）水平的影响。参与者双侧下肢肌肉（股四头肌、胫骨前肌、腓肠肌）接受30分钟的NMES治疗。在NMES治疗前和治疗后，每隔15分钟采集一次血样，直至随访2小时，并将NMES治疗前水平的平均值与NMES治疗后的峰值和谷值进行比较。对于有显著变化的细胞因子，我们进行了重复测量线性回归分析。我们还测量了NMES治疗后的乳酸和肌酸激酶水平。
我们招募了9名符合条件的参与者。IL-6峰值较NMES治疗前平均值显著升高[0.65（0.89）至1.04（0.89）pg/ml，P = 0.001]，IL-1谷值[0.08（0.07）至0.02（0.02）pg/ml，P = 0.041]和TNF-α谷值[2.42（0.54）至2.16（0.59）pg/ml，P = 0.021]显著降低。在重复测量回归分析中，我们发现NMES治疗后整个120分钟期间平均IL-6值显著更高，NMES治疗后30分钟时平均IL-1值显著更高。IL-10峰值、IL-6谷值、乳酸或肌酸激酶值无显著差异。
在9名健康人中，30分钟的NMES治疗在时间上与细胞因子变化相关，类似于主动运动的效果，可能介导了NMES对减轻肌肉无力的观察到的效果。
