O'Connor Gregory, Knecht Leslie D, Salgado Nelson, Strobel Sebastian, Pasini Patrizia, Daunert Sylvia
Department of Biochemistry and Molecular Biology, University of Miami, Miller School of Medicine, Miami, FL, 33136, USA.
Department of Chemistry, University of Miami, Miami, FL, 33146, USA.
Adv Biochem Eng Biotechnol. 2015 Oct 17. doi: 10.1007/10_2015_337.
Genetically engineered bacterial whole-cell biosensors are powerful tools that take advantage of bacterial proteins and pathways to allow for detection of a specific analyte. These biosensors have been employed for a broad range of applications, including the detection of bacterial quorum-sensing molecules (QSMs). Bacterial QSMs are the small molecules bacteria use for population density-dependent communication, a process referred to as quorum sensing (QS). Various research groups have investigated the presence of QSMs, including N-acyl homoserine lactones (AHLs) and autoinducer-2 (AI-2), in physiological samples in attempts to enhance our knowledge of the role of bacteria and QS in disease states. Continued studies in these fields may allow for improved patient care and therapeutics based upon QSMs. Furthermore, bacterial whole-cell biosensors have elucidated the roles of some antibiotics as QS agonists and antagonists. Graphical Abstract.
基因工程细菌全细胞生物传感器是利用细菌蛋白质和信号通路来检测特定分析物的强大工具。这些生物传感器已被广泛应用于包括检测细菌群体感应分子（QSMs）在内的众多领域。细菌QSMs是细菌用于群体密度依赖性通讯的小分子，这一过程称为群体感应（QS）。各个研究小组都在生理样本中研究了QSMs的存在情况，包括N-酰基高丝氨酸内酯（AHLs）和自诱导物-2（AI-2），以增进我们对细菌和QS在疾病状态中作用的了解。这些领域的持续研究可能会基于QSMs实现更好的患者护理和治疗。此外，细菌全细胞生物传感器还阐明了一些抗生素作为QS激动剂和拮抗剂的作用。图形摘要。
