特发性快速眼动睡眠行为障碍中的肠神经系统α-突触核蛋白免疫反应性
Enteric nervous system α-synuclein immunoreactivity in idiopathic REM sleep behavior disorder.
作者信息
Sprenger Fabienne S, Stefanova Nadia, Gelpi Ellen, Seppi Klaus, Navarro-Otano Judith, Offner Felix, Vilas Dolores, Valldeoriola Francesc, Pont-Sunyer Claustre, Aldecoa Iban, Gaig Carles, Gines Angels, Cuatrecasas Miriam, Högl Birgit, Frauscher Birgit, Iranzo Alex, Wenning Gregor K, Vogel Wolfgang, Tolosa Eduardo, Poewe Werner
机构信息
From the Departments of Neurology (F.S.S., N.S., K.S., B.H., B.F., G.K.W., W.P.) and Gastroenterology (W.V.), Medical University of Innsbruck, Austria; Neurological Tissue Bank of the Biobanc-Hospital Clinic de Barcelona (E.G.), IDIBAPS; the Departments of Neurology (J.N.-O., D.V., F.V., C.P.-S., C.G., A.I., E.T.), Pathology (I.A., M.C.), and Gastroenterology (A.G.), Hospital Clinic de Barcelona, Spain; and the Institute of Pathology (F.O.), Academic Teaching Hospital, Feldkirch, Austria.
出版信息
Neurology. 2015 Nov 17;85(20):1761-8. doi: 10.1212/WNL.0000000000002126. Epub 2015 Oct 16.
OBJECTIVE
To investigate the expression of α-synuclein in colonic biopsies of patients with idiopathic REM sleep behavior disorder (iRBD) and address if α-synuclein immunostaining of tissue obtained via colonic biopsies holds promise as a diagnostic biomarker for prodromal Parkinson disease (PD).
METHODS
Patients with iRBD, patients with PD, and healthy controls were prospectively recruited to undergo colonic biopsies for comparison of α-synuclein immunoreactivity patterns between the groups by using 2 different antibodies.
RESULTS
There was no difference in colonic mucosal and submucosal immunostaining between groups using the 15G7 α-synuclein antibody, which was found in almost all participants enrolled in this study. By contrast, immunostaining for serine 129-phosphorylated α-synuclein (pSyn) in submucosal nerve fibers or ganglia was found in none of 14 controls but was observed in 4 of 17 participants with iRBD and 1 out of 19 patients with PD.
CONCLUSIONS
The present findings of pSyn immunostaining of colonic biopsies in a substantial proportion of iRBD participants raise the possibility that this tissue marker may be a suitable candidate to study further as a prodromal PD marker in at-risk cohorts.
目的
研究α-突触核蛋白在特发性快速眼动睡眠行为障碍（iRBD）患者结肠活检组织中的表达，并探讨通过结肠活检获得的组织进行α-突触核蛋白免疫染色是否有望作为帕金森病（PD）前驱期的诊断生物标志物。
方法
前瞻性招募iRBD患者、PD患者和健康对照者，进行结肠活检，使用两种不同抗体比较各组间α-突触核蛋白免疫反应模式。
结果
使用15G7α-突触核蛋白抗体时，各组结肠黏膜和黏膜下层免疫染色无差异，该抗体在本研究纳入的几乎所有参与者中均有发现。相比之下，14名对照者的黏膜下神经纤维或神经节中均未发现丝氨酸129磷酸化α-突触核蛋白（pSyn）免疫染色，但在17名iRBD参与者中有4名观察到，在19名PD患者中有1名观察到。
结论
本研究中相当一部分iRBD参与者结肠活检组织pSyn免疫染色的结果提示，这种组织标志物有可能作为高危人群中PD前驱期标志物进一步深入研究。
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