Riard P, Hostein J, Croize J, Bourguignon G, Le Marc'Hadour F, Faure H, Fournet J
Service de Gastroentérologie et d'Hépatologie, Hópital A. Michallon, Grenoble.
Gastroenterol Clin Biol. 1989 Jan;13(1):8-13.
The aims of this prospective study were a) to evaluate the diagnostic value of the urease test for the detection of C. pylori in gastric biopsy specimens, b) to specify the prevalence of C. pylori in a sample of 74 patients from the Grenoble area undergoing upper gastrointestinal endoscopy, c) to analyze the density of bacteria according to the biopsy site (antrum, body, edges of ulcer), d) to demonstrate any possible correlation between the histologic state of the antral and body mucosa and the presence of C. pylori. An antral biopsy was taken for the urease test during endoscopy. Biopsies were also taken from the body, the antrum and the edges of gastric or duodenal ulcers for bacterial and histologic studies, and urease test in the bacterial laboratory. The sensitivity and the specificity of the urease test during endoscopy varied according to the delay in observation of the color change. They were 0.81 and 0.84, respectively, at 2 h 30. The sensitivity and specificity of the urease test in the bacterial laboratory were 0.67 and 0.95, respectively, for the same delay. The global prevalence of C. pylori was 51 p. 100: it was 42 p. 100 in the absence of ulcer, 67 p. 100 in the presence of gastric ulcer, and 71 p. 100 in the presence of duodenal ulcer (p less than 0.05 compared to the group without ulcer).(ABSTRACT TRUNCATED AT 250 WORDS)
a）评估尿素酶试验在胃活检标本中检测幽门螺杆菌的诊断价值；b）明确格勒诺布尔地区74例接受上消化道内镜检查患者样本中幽门螺杆菌的患病率；c）根据活检部位（胃窦、胃体、溃疡边缘）分析细菌密度；d）证明胃窦和胃体黏膜的组织学状态与幽门螺杆菌的存在之间是否存在任何可能的相关性。在内镜检查期间采集胃窦活检标本进行尿素酶试验。还从胃体、胃窦以及胃或十二指肠溃疡边缘采集活检标本进行细菌学和组织学研究，并在细菌实验室进行尿素酶试验。内镜检查时尿素酶试验的敏感性和特异性根据观察颜色变化的延迟时间而有所不同。在2小时30分时，它们分别为0.81和0.84。在细菌实验室中，相同延迟时间下尿素酶试验的敏感性和特异性分别为0.67和0.95。幽门螺杆菌的总体患病率为51%：无溃疡时为42%，有胃溃疡时为67%，有十二指肠溃疡时为71%（与无溃疡组相比，p<0.05）。（摘要截断于250字）
