Leonhäuser D, Vogt M, Tolba R H, Grosse J O
Department of Urology, RWTH Aachen University Hospital, Aachen, Germany.
Interdisciplinary Center for Clinical Research IZKF Aachen, RWTH Aachen University Hospital, Aachen, Germany.
J Biomater Appl. 2016 Feb;30(7):961-73. doi: 10.1177/0885328215610824. Epub 2015 Oct 15.
The aging society has a deep impact on patient care in urology. The number of patients in need of partial or whole bladder wall replacement is increasing simultaneously with the number of cancer incidents. Therefore, urological research requires a model of bladder wall replacement in adult and elderly people. Two types of porcine collagen I/III scaffolds were used in vitro for comparison of cell growth of two different pig breeds at different growth stages. Scaffolds were characterised with scanning electron and laser scanning microscopy. Urothelial and detrusor smooth muscle cells were isolated from 15 adult Göttingen minipigs and 15 juvenile German Landrace pigs. Growth behaviour was examined in cell culture and seeded onto the collagen scaffolds via immunohistochemistry, two-photon laser scanning microscopy and a viability assay. The collagen scaffolds showed different structured surfaces which are appropriate for seeding of the two different cell types. Moisturisation of the scaffolds resulted in a change of the structure. Cell growth of German Landrace urothelial cells and smooth muscle cells was significantly higher than cell growth of the Göttingen minipig cells. Seeding of scaffolds with both cell types from both pig races was possible which could be shown by immunohistochemistry and two-photon laser scanning microscopy. Growth behaviour on the scaffolds was significantly increased for the German Landrace compared to Göttingen minipig. Nevertheless, seeding with the adult Göttingen minipig cells resulted in a closed layer on the surface and urothelial cells and smooth muscle cells showed increasing growth until day 14. The results show that these collagen scaffolds are adequate for the seeding with vesical cells. Moreover, they seem appropriate for the use as an in vitro model for the adult or elderly as the cells of the adult Göttingen minipig too, show good growth behaviour.
老龄化社会对泌尿外科的患者护理产生了深远影响。需要部分或全部膀胱壁置换的患者数量与癌症发病率同步增加。因此，泌尿外科研究需要一种针对成年人和老年人的膀胱壁置换模型。两种类型的猪I/III型胶原蛋白支架在体外用于比较两种不同猪品种在不同生长阶段的细胞生长情况。通过扫描电子显微镜和激光扫描显微镜对支架进行表征。从15只成年哥廷根小型猪和15只幼年德国长白猪中分离出尿路上皮细胞和平滑肌细胞。在细胞培养中检查生长行为，并通过免疫组织化学、双光子激光扫描显微镜和活力测定将细胞接种到胶原蛋白支架上。胶原蛋白支架显示出不同的结构表面，适合两种不同细胞类型的接种。支架的保湿导致结构发生变化。德国长白猪尿路上皮细胞和平滑肌细胞的生长明显高于哥廷根小型猪细胞的生长。通过免疫组织化学和双光子激光扫描显微镜可以证明，两种猪品种的两种细胞类型都可以接种到支架上。与哥廷根小型猪相比，德国长白猪在支架上的生长行为明显增加。然而，接种成年哥廷根小型猪细胞导致表面形成封闭层，尿路上皮细胞和平滑肌细胞在第14天前生长不断增加。结果表明，这些胶原蛋白支架适合接种膀胱细胞。此外，它们似乎适合用作成人或老年人的体外模型，因为成年哥廷根小型猪的细胞也表现出良好的生长行为。
