Bartholomew Nathan R, Burdett Jacob M, VandenBrooks John M, Quinlan Michael C, Call Gerald B
Arizona College of Osteopathic Medicine (AZCOM), Midwestern University, Glendale, AZ, 85308, USA.
Department of Physiology, AZCOM, Midwestern University, Glendale, AZ 85308, USA.
Sci Rep. 2015 Oct 19;5:15298. doi: 10.1038/srep15298.
Laboratories that study Drosophila melanogaster or other insects commonly use carbon dioxide (CO2) anaesthesia for sorting or other work. Unfortunately, the use of CO2 has potential unwanted physiological effects, including altered respiratory and muscle physiology, which impact motor function behaviours. The effects of CO2 at different levels and exposure times were examined on the subsequent recovery of motor function as assessed by climbing and flight assays. With as little as a five minute exposure to 100% CO2, D. melanogaster exhibited climbing deficits up to 24 hours after exposure. Any exposure length over five minutes produced climbing deficits that lasted for days. Flight behaviour was also impaired following CO2 exposure. Overall, there was a positive correlation between CO2 exposure length and recovery time for both behaviours. Furthermore, exposure to as little as 65% CO2 affected the motor capability of D. melanogaster. These negative effects are due to both a CO2-specific mechanism and an anoxic effect. These results indicate a heretofore unconsidered impact of CO2 anaesthesia on subsequent behavioural tests revealing the importance of monitoring and accounting for CO2 exposure when performing physiological or behavioural studies in insects.
研究黑腹果蝇或其他昆虫的实验室通常使用二氧化碳（CO₂）麻醉来进行分类或其他工作。不幸的是，使用CO₂存在潜在的不良生理影响，包括呼吸和肌肉生理的改变，这会影响运动功能行为。通过攀爬和飞行试验评估了不同浓度和暴露时间的CO₂对随后运动功能恢复的影响。仅暴露于100% CO₂五分钟，黑腹果蝇在暴露后长达24小时就表现出攀爬能力缺陷。任何超过五分钟的暴露时间都会导致持续数天的攀爬能力缺陷。CO₂暴露后飞行行为也受到损害。总体而言，两种行为的CO₂暴露时间和恢复时间之间存在正相关。此外，仅暴露于65%的CO₂就会影响黑腹果蝇的运动能力。这些负面影响是由特定于CO₂的机制和缺氧效应共同导致的。这些结果表明，CO₂麻醉对后续行为测试有此前未被考虑到的影响，这揭示了在对昆虫进行生理或行为研究时监测和考虑CO₂暴露的重要性。
