Marcos Josep, Pozo Oscar J
Department of Experimental & Health Sciences, Universitat Pompeu Fabra, Doctor Aiguader 88, 08003 Barcelona, Spain.
Toxicology Department, LabcoDiagnostics, Verge de Guadalupe 18,08950 Esplugues de Llobregat, Spain.
Bioanalysis. 2015 Oct;7(19):2515-36. doi: 10.4155/bio.15.176. Epub 2015 Oct 19.
The determination of steroids in biological samples is essential in different areas of knowledge. MS combined with either GC or LC is considered the best analytical technique for specific and sensitive determinations. However, due to the physicochemical properties of some steroids, and the low concentrations found in biological samples, the formation of a derivative prior to their analysis is required. In GC-MS determinations, derivatization is needed for generating volatile and thermally stable compounds. The improvement in terms of stability and chromatographic retention are the main reasons for selecting the derivatization agent. On the other hand, derivatization is not compulsory in LC-MS analyses and the derivatization is typically used for improving the ionization and therefore the overall sensitivity achieved.
生物样品中类固醇的测定在不同知识领域都至关重要。质谱联用气相色谱或液相色谱被认为是进行特异性和灵敏性测定的最佳分析技术。然而，由于某些类固醇的物理化学性质以及生物样品中发现的低浓度，在分析之前需要形成衍生物。在气相色谱 - 质谱测定中，需要进行衍生化以生成挥发性和热稳定的化合物。稳定性和色谱保留方面的改善是选择衍生化试剂的主要原因。另一方面，在液相色谱 - 质谱分析中衍生化并非必需，衍生化通常用于改善离子化，从而提高整体灵敏度。
