Zhijiang Cai, Yi Xu, Haizheng Yang, Jia Jianru, Liu Yuanpei
School of Textiles, Tianjin Polytechnic University, Tianjin 300387, China; State Key Laboratory of Hollow Fiber Membrane Material and Processes, No 399 BingShuiXi Street, XiQing District, Tianjin, China, 300387.
School of Textiles, Tianjin Polytechnic University, Tianjin 300387, China.
Mater Sci Eng C Mater Biol Appl. 2016 Jan 1;58:757-67. doi: 10.1016/j.msec.2015.09.048. Epub 2015 Sep 14.
Poly(hydroxybutyrate) (PHB)/cellulose acetate (CA) blend nanofiber scaffolds were fabricated by electrospinning using the blends of chloroform and DMF as solvent. The blend nanofiber scaffolds were characterized by SEM, FTIR, XRD, DSC, contact angle and tensile test. The blend nanofibers exhibited cylindrical, uniform, bead-free and random orientation with the diameter ranged from 80-680 nm. The scaffolds had very well interconnected porous fibrous network structure and large aspect surface areas. It was found that the presence of CA affected the crystallization of PHB due to formation of intermolecular hydrogen bonds, which restricted the preferential orientation of PHB molecules. The DSC result showed that the PHB and CA were miscible in the blend nanofiber. An increase in the glass transition temperature was observed with increasing CA content. Additionally, the mechanical properties of blend nanofiber scaffolds were largely influenced by the weight ratio of PHB/CA. The tensile strength, yield strength and elongation at break of the blend nanofiber scaffolds increased from 3.3 ± 0.35 MPa, 2.8 ± 0.26 MPa, and 8 ± 0.77% to 5.05 ± 0.52 MPa, 4.6 ± 0.82 MPa, and 17.6 ± 1.24% by increasing PHB content from 60% to 90%, respectively. The water contact angle of blend nanofiber scaffolds decreased about 50% from 112 ± 2.1° to 60 ± 0.75°. The biodegradability was evaluated by in vitro degradation test and the results revealed that the blend nanofiber scaffolds showed much higher degradation rates than the neat PHB. The cytocompatibility of the blend nanofiber scaffolds was preliminarily evaluated by cell adhesion studies. The cells incubated with PHB/CA blend nanofiber scaffold for 48 h were capable of forming cell adhesion and proliferation. It showed much better biocompatibility than pure PHB film. Thus, the prepared PHB/CA blend nanofiber scaffolds are bioactive and may be more suitable for cell proliferation suggesting that these scaffolds can be used for wound dressing or tissue-engineering scaffolds.
采用氯仿和N,N - 二甲基甲酰胺（DMF）的混合溶剂，通过静电纺丝制备了聚羟基丁酸酯（PHB）/醋酸纤维素（CA）共混纳米纤维支架。通过扫描电子显微镜（SEM）、傅里叶变换红外光谱（FTIR）、X射线衍射（XRD）、差示扫描量热法（DSC）、接触角测量和拉伸试验对共混纳米纤维支架进行了表征。共混纳米纤维呈圆柱形，均匀、无珠且取向随机，直径范围为80 - 680纳米。支架具有相互连通性良好的多孔纤维网络结构和较大的比表面积。研究发现，由于分子间氢键的形成，CA的存在影响了PHB的结晶，这限制了PHB分子的择优取向。DSC结果表明，PHB和CA在共混纳米纤维中是可混溶的。随着CA含量的增加，玻璃化转变温度升高。此外，但共混纳米纤维支架的力学性能在很大程度上受PHB/CA重量比的影响。通过将PHB含量从60%增加到90%，共混纳米纤维支架的拉伸强度、屈服强度和断裂伸长率分别从3.3±0.35兆帕、2.8±0.26兆帕和8±0.77%提高到5.05±0.52兆帕、4.6±（此处原文有误，应为4.6±0.82）兆帕和17.6±1.24%。共混纳米纤维支架的水接触角从112±2.1°下降到60±0.75°，下降了约50%。通过体外降解试验评估了生物降解性，结果表明，共混纳米纤维支架的降解速率比纯PHB高得多。通过细胞黏附研究对共混纳米纤维支架的细胞相容性进行了初步评估。在PHB/CA共混纳米纤维支架上培养48小时的细胞能够形成细胞黏附和增殖。它显示出比纯PHB膜更好的生物相容性。因此，制备的PHB/CA共混纳米纤维支架具有生物活性，可能更适合细胞增殖，表明这些支架可用于伤口敷料或组织工程支架。
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