Zhong Ping, Fan Xian-ming, Huang Cheng-liang, Wang Wen-jun, Zhan Xiao-qin, He Xiao-peng
Sichuan Da Xue Xue Bao Yi Xue Ban. 2015 Jul;46(4):533-6.
To investigate the regulatory effect of B cell activating transcription factor (BATF) on acute airway inflammation and its association with retinoic acid orphan nuclear receptors gammat (RORyt) in asthmatic mice.
24 female BALB/c mice were randomly and equally divided into three groups (n 8): normal saline (NS) treated, asthma (AS) control and dexamethasone (DEX) treated. AS mice were sensitized and challenged with OVA to establish murine asthma model. Histological changes in lung tissues of the mice were observed by HE staining. Numbers of white blood cell (WBC), polymorphonuclear leukocyte (PMN) and eosinophils (EOS) in the bronchoalveolar lavage fluid (BALF) of the mice were counted. The concentration of interleukin-17 (IL-17) in BALF was measured by ELISA. Quantitative real-time PCR (RT-PCR) was performed to assess the mRNA expressions of BATF, IL-17 and RORγt in the lung tissues.
The HE staining showed a higher level of inflammatory cell infiltration around the bronchi of AS mice compared with those treated with NS, predominantly in the forms of EOS, PMN and lymphocytes. The AS and DEX treated mice had higher levels of EOS, PMN, WBC and mRNA expressions of BATF, IL-17 and RORγt in BALF than those treated with NS (P < 0.05). DEX reduced the levels of EOS, PMN, WBC and IL-17 in BALF significantly (P < 0.05). The mRNA expression of BATF in lung tissues of mice was positively correlated with the expression of IL-17, RORγt and the counts of WBC,EOS and PMN in BALF (P < 0.05).
Asthmatic mice have increased expressions of BATF, IL-17 and RORγt in bronchial and lung tissues. BATF can, through regulating the secretion of Th17 cells, readjust the airway inflammatory. The regulatory function may take effect through synergy with RORγt .
探讨B细胞活化转录因子（BATF）对哮喘小鼠急性气道炎症的调节作用及其与维甲酸孤儿核受体γt（RORyt）的关系。
将24只雌性BALB/c小鼠随机均分为三组（n = 8）：生理盐水（NS）处理组、哮喘（AS）对照组和地塞米松（DEX）处理组。用卵清蛋白（OVA）对AS小鼠进行致敏和激发以建立小鼠哮喘模型。通过苏木精-伊红（HE）染色观察小鼠肺组织的组织学变化。计数小鼠支气管肺泡灌洗液（BALF）中的白细胞（WBC）、多形核白细胞（PMN）和嗜酸性粒细胞（EOS）数量。用酶联免疫吸附测定（ELISA）法检测BALF中白细胞介素-17（IL-17）的浓度。采用定量实时聚合酶链反应（RT-PCR）评估肺组织中BATF、IL-17和RORγt的mRNA表达。
HE染色显示，与NS处理组相比，AS小鼠支气管周围炎症细胞浸润水平更高，主要表现为EOS、PMN和淋巴细胞浸润。AS组和DEX处理组小鼠BALF中的EOS、PMN、WBC水平以及BATF、IL-17和RORγt的mRNA表达均高于NS处理组（P < 0.05）。DEX显著降低了BALF中EOS、PMN、WBC和IL-17的水平（P < 0.05）。小鼠肺组织中BATF的mRNA表达与IL-17、RORγt的表达以及BALF中WBC、EOS和PMN的计数呈正相关（P < 0.05）。
哮喘小鼠支气管和肺组织中BATF、IL-17和RORγt的表达增加。BATF可通过调节Th17细胞的分泌来重新调整气道炎症。该调节功能可能通过与RORγt协同作用而发挥效应。
