溶血磷脂酸调节牛类固醇生成黄体细胞中的前列腺素信号传导。
Lysophosphatidic acid modulates prostaglandin signalling in bovine steroidogenic luteal cells.
作者信息
Kowalczyk-Zieba Ilona, Boruszewska Dorota, Sinderewicz Emilia, Grycmacher Katarzyna, Woclawek-Potocka Izabela
机构信息
Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland.
Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland.
出版信息
Prostaglandins Other Lipid Mediat. 2015 Sep;121(Pt B):218-26. doi: 10.1016/j.prostaglandins.2015.10.002. Epub 2015 Oct 19.
We examined whether lysophosphatidic acid affects prostaglandin biosynthesis, transport, and signalling in bovine steroidogenic luteal cells. The aim of the present study was to determine the influence of LPA on PGE2 and PGF2α synthesis and on the expression of enzymes involved in PG biosynthesis (PTGS2, mPGES-1, cPGES, mPGES-2, PGFS and 9-KPR), prostaglandin transporter (PGT), and prostaglandin receptors (EP1, EP2, EP3, EP4 and FP) in bovine steroidogenic luteal cells. We found that LPA inhibited PGF2α synthesis in steroidogenic luteal cells. Moreover, LPA increased mPGES1 and cPGES and decreased PGFS expression in cultured bovine steroidogenic luteal cells. Additionally, LPA stimulated EP2 and EP4 receptor and PGT expression. This study suggests that LPA activity in the bovine CL directs the physiological intraluteal balance between the two main prostanoids towards luteotropic PGE2.
我们研究了溶血磷脂酸是否会影响牛类固醇生成黄体细胞中前列腺素的生物合成、转运及信号传导。本研究的目的是确定溶血磷脂酸对牛类固醇生成黄体细胞中前列腺素E2和前列腺素F2α合成以及参与前列腺素生物合成的酶（前列腺素内过氧化物合酶2、微粒体前列腺素E合酶-1、胞质型前列腺素E合酶、微粒体前列腺素E合酶-2、前列腺素F合酶和9-酮前列腺素还原酶）、前列腺素转运体及前列腺素受体（EP1、EP2、EP3、EP4和FP）表达的影响。我们发现溶血磷脂酸抑制了类固醇生成黄体细胞中前列腺素F2α的合成。此外，溶血磷脂酸增加了培养的牛类固醇生成黄体细胞中微粒体前列腺素E合酶1和胞质型前列腺素E合酶的表达，并降低了前列腺素F合酶的表达。另外，溶血磷脂酸刺激了EP2和EP4受体以及前列腺素转运体的表达。本研究表明，牛黄体中溶血磷脂酸的活性将两种主要前列腺素之间的生理黄体内平衡导向促黄体生成的前列腺素E2。
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