Song Xiaofeng, Wei Haichao, Cheng Wen, Yang Suxin, Zhao Yanxiu, Li Xuan, Luo Da, Zhang Hui, Feng Xianzhong
Key Laboratory of Systems Biology in Universities of Shandong, College of Life Science, Shandong Normal University, 250014 Jinan, China.
Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, 130102 Changchun, China.
G3 (Bethesda). 2015 Oct 19;5(12):2793-9. doi: 10.1534/g3.115.022780.
Soybean [Glycine max (L.) Merrill] is an important crop worldwide. In this study, a Chinese local soybean cultivar, Hedou 12, was resequenced by next generation sequencing technology to develop INsertion/DELetion (INDEL) markers for genetic mapping. 49,276 INDEL polymorphisms and 242,059 single nucleotide polymorphisms were detected between Hedou 12 and the Williams 82 reference sequence. Of these, 243 candidate INDEL markers ranging from 5-50 bp in length were chosen for validation, and 165 (68%) of them revealed polymorphisms between Hedou 12 and Williams 82. The validated INDEL markers were also tested in 12 other soybean cultivars. The number of polymorphisms in the pairwise comparisons of 14 soybean cultivars varied from 27 to 165. To test the utility of these INDEL markers, they were used to perform genetic mapping of a crinkly leaf mutant, and the CRINKLY LEAF locus was successfully mapped to a 360 kb region on chromosome 7. This research shows that high-throughput sequencing technologies can facilitate the development of genome-wide molecular markers for genetic mapping in soybean.
大豆[Glycine max (L.) Merrill]是全球重要的农作物。在本研究中，利用新一代测序技术对中国地方大豆品种合豆12进行重测序，以开发用于遗传图谱构建的插入/缺失（INDEL）标记。在合豆12与Williams 82参考序列之间检测到49276个INDEL多态性位点和242059个单核苷酸多态性位点。其中，选择了243个长度在5 - 50 bp之间的候选INDEL标记进行验证，其中165个（68%）在合豆12和Williams 82之间表现出多态性。经过验证的INDEL标记也在其他12个大豆品种中进行了测试。14个大豆品种两两比较中的多态性位点数在27到165之间变化。为了测试这些INDEL标记的实用性，利用它们对一个皱叶突变体进行遗传图谱构建，并且成功地将CRINKLY LEAF基因座定位到7号染色体上一个360 kb的区域。本研究表明，高通量测序技术有助于大豆遗传图谱构建的全基因组分子标记的开发。
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