Yu Hui, Wang Qiushi, Zhang Zhirui, Wu Tao, Yang Xinjing, Zhu Xiaobin, Ye Yongheng, Leng Jiantian, Yang Suxin, Feng Xianzhong
Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, China.
College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, China.
Front Plant Sci. 2022 May 26;13:892077. doi: 10.3389/fpls.2022.892077. eCollection 2022.
The generation of oxygen and organic matter in plants mainly depends on photosynthesis, which directly affects plant growth and development. The chloroplast is the main organelle in which photosynthesis occurs. In this study, a pale green leaf 3-1 () mutant was isolated from the soybean mutagenized population. The mutant presented with decreased chlorophyll contents, reduced chloroplast stroma thylakoids, reduced yields, and decreased numbers of pods per plant. Bulked segregant analysis (BSA) together with map-based cloning revealed a single-nucleotide non-synonymous mutation at the 341st nucleotide of the first exon of the chloroplast development-related gene. The phenotype of the knockout plants was the same as that of the mutant. The gene was highly expressed in the leaves at various developmental stages, and its protein was localized to the inner chloroplast membrane. Split luciferase complementation assays and coimmunoprecipitation (co-IP) experiments revealed that GmTic110a interacted with GmTic20, GmTic40a, and GmTic40b in tobacco leaves. These results indicated that the gene plays an important role in chloroplast development.
植物中氧气和有机物的产生主要依赖于光合作用，光合作用直接影响植物的生长发育。叶绿体是进行光合作用的主要细胞器。在本研究中，从大豆诱变群体中分离出一个淡绿叶3-1（）突变体。该突变体叶绿素含量降低，叶绿体基质类囊体减少，产量降低，单株荚数减少。混合分组分析法（BSA）结合图位克隆揭示，叶绿体发育相关基因第一个外显子第341个核苷酸处存在一个单核苷酸非同义突变。敲除植株的表型与突变体相同。该基因在各个发育阶段的叶片中均高表达，其蛋白定位于叶绿体内膜。双荧光素酶互补分析和免疫共沉淀（co-IP）实验表明，GmTic110a在烟草叶片中与GmTic20、GmTic40a和GmTic40b相互作用。这些结果表明该基因在叶绿体发育中起重要作用。
