钻石黑范贫血的Rpl11缺陷斑马鱼模型的转录组分析。
Transcriptome analysis of Rpl11-deficient zebrafish model of Diamond-Blackfan Anemia.
作者信息
Zhang Zhaojun, Jia Haibo, Zhang Qian, Wan Yang, Song Binfeng, Jia Qiong, Liu Hanzhi, Zhu Xiaofan, Fang Xiangdong
机构信息
CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China.
Key Laboratory of Molecular Biophysics of Ministry of Education, College of Life Science and Technology Center for Human Genome Research, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China.
出版信息
Genom Data. 2014 Jun 17;2:173-5. doi: 10.1016/j.gdata.2014.06.008. eCollection 2014 Dec.
To comprehensively reflect the roles of Rpl11 on the transcriptome of zebrafish model of Diamond-Blackfan Anemia (DBA), we performed whole-genome transcriptome sequencing on the Illumina Hi-Seq 2000 sequencing platform. Two different transcriptomes of zebrafish Rpl11-deficient and control Morpholino (Mo) embryos were collected and analyzed. The experimental design and methods, including sample preparation, RNA-Seq data evaluation and treatment, were described in details so that representative high-throughput sequencing data were acquired for assessing the actual impacts of Rpl11 on zebrafish embryos. We provided the accession number GSE51326 for easy access to the database.
为全面反映核糖体蛋白L11（Rpl11）在钻石黑范贫血（DBA）斑马鱼模型转录组中的作用，我们在Illumina Hi-Seq 2000测序平台上进行了全基因组转录组测序。收集并分析了斑马鱼Rpl11缺陷型和对照吗啉代（Mo）胚胎的两种不同转录组。详细描述了实验设计和方法，包括样品制备、RNA测序数据评估和处理，以便获得具有代表性的高通量测序数据，用于评估Rpl11对斑马鱼胚胎的实际影响。我们提供了登录号GSE51326，以便于访问数据库。
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