Thakare Dhiraj, Yang Ruolin, Steffen Joshua G, Zhan Junpeng, Wang Dongfang, Clark Richard M, Wang Xiangfeng, Yadegari Ramin
School of Plant Sciences, University of Arizona, Tucson, AZ 85721, USA.
Department of Biology, University of Utah, Salt Lake City, UT 84112, USA.
Genom Data. 2014 Aug 7;2:242-5. doi: 10.1016/j.gdata.2014.07.003. eCollection 2014 Dec.
Endosperm is a product of double fertilization, and provides nutrients and signals to the embryo during seed development in flowering plants. Early stages of endosperm development are critical for the development of its storage capacity through synthesis and accumulation of starch and storage proteins. Here we report on the isolation and sequencing of mRNAs from the central portion of the starchy endosperm of Zea mays (maize) B73 at 6 days after pollination. We detected a high level of correlation among the four biological replicates of RNAs isolated using laser-capture microdissection of the cell type. Because the assayed developmental stage precedes the synthesis and accumulation of the major storage proteins and starch in the endosperm, our dataset likely include mRNAs for genes that are involved in control and establishment of these storage programs. The mRNA-Seq data has been deposited in Gene Expression Omnibus (accession number GSE58504).
胚乳是双受精的产物，在开花植物种子发育过程中为胚提供营养和信号。胚乳发育的早期阶段对于通过淀粉和贮藏蛋白的合成与积累来发展其贮藏能力至关重要。本文报道了授粉后6天从玉米（Zea mays）B73的粉质胚乳中央部分分离mRNA并进行测序的结果。我们检测到通过激光捕获显微切割该细胞类型分离的RNA的四个生物学重复之间具有高度相关性。由于所分析的发育阶段先于胚乳中主要贮藏蛋白和淀粉的合成与积累，我们的数据集可能包含参与这些贮藏程序控制和建立的基因的mRNA。mRNA测序数据已存入基因表达综合数据库（登录号GSE58504）。
