通过将生物素化寡核苷酸连接到基因组区域来绘制基因组双链断裂图谱：对人类核糖体DNA单位获得的数据进行分析。
Mapping of genomic double-strand breaks by ligation of biotinylated oligonucleotides to forum domains: Analysis of the data obtained for human rDNA units.
作者信息
Tchurikov N A, Kretova O V, Fedoseeva D M, Chechetkin V R, Gorbacheva M A, Karnaukhov A A, Kravatskaya G I, Kravatsky Y V
机构信息
Engelhardt Institute of Molecular Biology, Moscow, Russia.
出版信息
Genom Data. 2014 Nov 12;3:15-8. doi: 10.1016/j.gdata.2014.10.024. eCollection 2015 Mar.
DNA double-strand breaks (DSBs) are associated with different physiological and pathological processes in different organisms. To understand the role of DSBs in multiple cellular mechanisms, a robust method for genome-wide mapping of chromosomal breaks at one-nucleotide resolution is required. Many years ago, we detected large DNA fragments migrating from DNA-agarose plugs in pulsed-field gels, which we named 'forum domains' [1,2]. Recently, we developed a method for genome-wide mapping of DSBs that produces these 50-150 kb DNA domains using microarrays or 454 sequencing (Tchurikov et al., 2011; 2013). Now we have used Illumina sequencing to map DSBs in repetitive rDNA units in human HEK293T cells. Here we describe in detail the experimental design and bioinformatics analysis of the data deposited in the Gene Expression Omnibus with accession number GSE49302 and associated with the study published in the Journal of Molecular Cell Biology (Tchurikov et al., 2014).
DNA双链断裂（DSB）在不同生物体中与不同的生理和病理过程相关。为了了解DSB在多种细胞机制中的作用，需要一种强大的方法以单核苷酸分辨率对全基因组范围内的染色体断裂进行定位。许多年前，我们在脉冲场凝胶中检测到从DNA琼脂糖凝胶块迁移出的大片段DNA，我们将其命名为“论坛结构域”[1,2]。最近，我们开发了一种用于全基因组范围内DSB定位的方法，该方法使用微阵列或454测序产生这些50 - 150 kb的DNA结构域（Tchurikov等人，2011年；2013年）。现在我们使用Illumina测序对人类HEK293T细胞中重复的核糖体DNA（rDNA）单元中的DSB进行定位。在这里，我们详细描述了 deposited in the Gene Expression Omnibus中登录号为GSE49302的数据的实验设计和生物信息学分析，这些数据与发表在《分子细胞生物学杂志》上的研究相关（Tchurikov等人，2014年）。
需注意，原文中“deposited in the Gene Expression Omnibus”表述不太完整准确，可能是“deposited in the Gene Expression Omnibus database”之类，这里按现有原文翻译。
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