拟南芥热胁迫下无帽mRNA的全基因组分析。
Genome-wide analysis of uncapped mRNAs under heat stress in Arabidopsis.
作者信息
Gutierrez-Beltran Emilio
机构信息
Department of Plant Biology, Uppsala BioCenter, Swedish University of Agricultural Sciences and Linnean Center for Plant Biology, SE-75007 Uppsala, Sweden.
出版信息
Genom Data. 2015 May 4;5:7-8. doi: 10.1016/j.gdata.2015.04.020. eCollection 2015 Sep.
Recently, we have showed that Tudor Staphylococcal Nuclease (TSN or Tudor-SN) proteins (TSN1 and TSN2) are localized in cytoplasmic messenger ribonucleoprotein (mRNP) complexes called stress granules (SG) and processing bodies (PB) under heat stress in Arabidopsis. One of the primary functions of these mRNP complexes is mRNA decay, which generates uncapped mRNAs by the action of endonucleases and decapping enzymes (Thomas et al., 2011) [1]. In order to figure out whether TSN proteins could be implicated in mRNA decay, we isolated uncapped and total mRNAs of Wild type (WT; Col and Ler) and TSN double knock-out (tsn1tsn2) seedlings grown under heat stress (39 °C for 40 min) and control (23 °C) conditions. Here, we provide the experimental procedure to reproduce the results (NCBI GEO accession number GSE63522) published by Gutierrez-Beltran et al. (2015) in The Plant Cell [2].
最近，我们发现拟南芥在热胁迫下，都铎葡萄球菌核酸酶（TSN或都铎-SN）蛋白（TSN1和TSN2）定位于细胞质信使核糖核蛋白（mRNP）复合物中，这些复合物被称为应激颗粒（SG）和加工小体（PB）。这些mRNP复合物的主要功能之一是mRNA降解，其通过核酸内切酶和脱帽酶的作用产生无帽mRNA（Thomas等人，2011年）[1]。为了弄清楚TSN蛋白是否与mRNA降解有关，我们分离了在热胁迫（39°C处理40分钟）和对照（23°C）条件下生长的野生型（WT；Col和Ler）和TSN双敲除（tsn1tsn2）幼苗的无帽mRNA和总mRNA。在这里，我们提供了实验步骤，以重现Gutierrez-Beltran等人（2015年）在《植物细胞》[2]上发表的结果（NCBI GEO登录号GSE63522）。
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