膀胱癌中WDR5调控基因的基因表达谱分析
Gene expression profiling of WDR5 regulated genes in bladder cancer.
作者信息
Chen Xu, Gu Peng, Li Kuiqing, Xie Weibin, Chen Changhao, Lin Tianxin, Huang Jian
机构信息
Department of Urology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China ; Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China.
Department of Urology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China.
出版信息
Genom Data. 2015 May 14;5:27-9. doi: 10.1016/j.gdata.2015.05.003. eCollection 2015 Sep.
WD repeat domain 5 (WDR5) plays an important role in various biological functions through the epigenetic regulation of gene transcription (Wysocka et al., 2005 [1]; Sandstrom et al., 2014[2]; Ang et al., 2011[3]). Recently, our study found that WDR5 was upregulated in bladder cancer tissues, promoted bladder cancer cell proliferation, self-renewal and chemoresistance to cisplatin in bladder cancer cells in vitro, and tumor growth in vivo (Chen et al., 2015). To gain a molecular understanding of the role of WDR5 in promoting bladder cancer, we performed a genome-wide analysis on WDR5 knockdown by microarray gene expression profiling. Here we provide detailed experimental methods and analysis for the microarray data, which have been deposited into Gene Expression Omnibus (GEO): GSE59132.
WD重复结构域5（WDR5）通过基因转录的表观遗传调控在各种生物学功能中发挥重要作用（维索茨卡等人，2005年[1]；桑德斯特伦等人，2014年[2]；安等人，2011年[3]）。最近，我们的研究发现WDR5在膀胱癌组织中上调，在体外促进膀胱癌细胞增殖、自我更新以及对顺铂的化疗耐药性，并在体内促进肿瘤生长（陈等人，2015年）。为了从分子水平了解WDR5在促进膀胱癌中的作用，我们通过微阵列基因表达谱分析对WDR5敲低进行了全基因组分析。在此，我们提供了微阵列数据的详细实验方法和分析，这些数据已存入基因表达综合数据库（GEO）：GSE59132。
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