头颈部鳞状细胞癌表达谱中选定 miRNA 表达的不可预测变化。
Unpredictable changes of selected miRNA in expression profile of HNSCC.
机构信息
Laboratory of Cancer Genetics, Greater Poland Cancer Center, Poznan, Poland.
European Center of Bioinformatics and Genomics (ECBaG), Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland.
出版信息
Cancer Biomark. 2016;16(1):55-64. doi: 10.3233/CBM-150540.
BACKGROUND
The necessity of prediction and treatment outcome improvement of HNSCC needs to find new biomarkers. miRNAs seem to be good candidate for that.
OBJECTIVE
Analysis of selected 5 miRNAs (let-7d, miR-18a, miR-21, miR-205 and miR-375) as potential biomarkers that allows to distinguish tumor and healthy tissue taken from HNSCC patients.
METHODS
Tumor and normal epithelial tissues were obtained from 75 HNSCC patients to analyze selected miRNAs.
RESULTS
Analysis indicated significant increase of miR-21 and miR-205 in tumor when compared with healthy tissue (p= 0.0069 and p= 0.0029, respectively). There was a significant correlation between let-7d and miR-18a. let-7d was down-regulated in 34.67% cases, miR-18a in 29.33%, miR-21 in 20%, miR-205 in 30.67% and miR-375 in 52% cases. At the same time over-expression of let-7d was detected in 18.67% cases, miR-18a in 22.67%, miR-21 in 48%, miR-205 in 41.33% and miR-375 in 52% cases. There was no correlation between miRNA expression and clinical data and the course of illness.
CONCLUSION
Our study indicated that miR-21 and miR-205 can be used to analyze the clarity of surgical margins and that concomitant changes in the expression of let-7 and miR-18a in tumor tissues might represent important future markers indicating the biology of HNSCC. These observations will help with developing personalization for HNSCC patients' treatment.
背景
预测和改善头颈部鳞状细胞癌（HNSCC）治疗效果的必要性需要寻找新的生物标志物。miRNA 似乎是一个很好的候选者。
目的
分析选定的 5 个 miRNA（let-7d、miR-18a、miR-21、miR-205 和 miR-375）作为潜在的生物标志物，用于区分来自 HNSCC 患者的肿瘤组织和健康组织。
方法
从 75 名 HNSCC 患者中获取肿瘤和正常上皮组织，以分析选定的 miRNA。
结果
分析表明，与健康组织相比，肿瘤组织中 miR-21 和 miR-205 的表达显著增加（p=0.0069 和 p=0.0029）。let-7d 和 miR-18a 之间存在显著相关性。let-7d 在 34.67%的病例中下调，miR-18a 在 29.33%的病例中下调，miR-21 在 20%的病例中下调，miR-205 在 30.67%的病例中上调，miR-375 在 52%的病例中上调。同时，在 18.67%的病例中检测到 let-7d 的过表达，在 22.67%的病例中检测到 miR-18a 的过表达，在 48%的病例中检测到 miR-21 的过表达，在 41.33%的病例中检测到 miR-205 的过表达，在 52%的病例中检测到 miR-375 的过表达。miRNA 表达与临床数据和病程无相关性。
结论
我们的研究表明，miR-21 和 miR-205 可用于分析手术切缘的清晰度，肿瘤组织中 let-7 和 miR-18a 表达的同时变化可能代表着重要的未来标志物，表明 HNSCC 的生物学特性。这些观察结果将有助于为 HNSCC 患者的治疗制定个性化方案。
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