中性粒细胞激活蛋白（NAP-1）对T淋巴细胞也具有趋化作用。
The neutrophil-activating protein (NAP-1) is also chemotactic for T lymphocytes.
作者信息
Larsen C G, Anderson A O, Appella E, Oppenheim J J, Matsushima K
机构信息
Laboratory of Molecular Immunoregulation, National Cancer Institute, Frederick, MD 21701.
出版信息
Science. 1989 Mar 17;243(4897):1464-6. doi: 10.1126/science.2648569.
T lymphocyte chemotactic factor (TCF) was purified to homogeneity from the conditioned media of phytohemagglutinin-stimulated human blood mononuclear leukocytes by a sequence of chromatography procedures. The amino-terminal amino acid sequence of the purified TCF showed identity with neutrophil-activating protein (NAP-1). Both TCF and recombinant NAP-1 (rNAP-1) were chemotactic for neutrophils and T lymphocytes in vitro supporting the identity of TCF with NAP-1. Injection of rNAP-1 into lymphatic drainage areas of lymph nodes in Fisher rats caused accelerated emigration of only lymphocytes in high endothelial venules. Intradermal injection of rNAP-1 caused dose-dependent accumulation of neutrophils and lymphocytes.
通过一系列色谱方法，从植物血凝素刺激的人血单核白细胞的条件培养基中纯化出均一的T淋巴细胞趋化因子（TCF）。纯化的TCF的氨基末端氨基酸序列与中性粒细胞激活蛋白（NAP-1）相同。TCF和重组NAP-1（rNAP-1）在体外对中性粒细胞和T淋巴细胞均具有趋化作用，这支持了TCF与NAP-1的同一性。将rNAP-1注射到Fisher大鼠淋巴结的淋巴引流区域，仅导致高内皮小静脉中的淋巴细胞加速迁出。皮内注射rNAP-1会导致中性粒细胞和淋巴细胞呈剂量依赖性聚集。
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