Nath Partha Pratim, Sarkar Kaushik, Mondal Mukti, Paul Goutam
Toxicology Unit, Environmental Physiology Division, Department of Physiology, University of Kalyani, West Bengal, 741235, India.
Environ Toxicol. 2016 Dec;31(12):2057-2067. doi: 10.1002/tox.22205. Epub 2015 Oct 23.
Metanil yellow (MY) is a most frequently used food color in West Bengal, India. The toxic effects of MY on the male reproductive system have been reported discriminately in animal models. The probable toxic effects of MY on female reproductive functions have not been reported till date. Therefore, this study was designed to examine the effect of MY on estrous cycle rhythmicity and ovarian folliculogenesis in female rats. Rats have been exposed to MY at three doses of 250, 500, 750 mg kgBW day for two exposure durations, 20 and 30 days. We observed significant changes in the number and duration of estrous cycle along with prominent cytoarchitectural changes in the cellular characteristics of vaginal smear of component phases of estrous cycle in a dose and duration-dependent manner in MY-treated rats compared to control rats. We also observed a significant decrease in serum follicle stimulating hormone (FSH), luteinizing hormone (LH), and estradiol levels in MY-treated rats. Further, the activities of some antioxidants enzymes in brain tissues of MY-treated rats were significantly decreased and the level of malondialdehyde (MDA), a marker of lipid peroxidation, in brain tissues of MY-treated rats was also significantly increased. The ovarian folliculogenesis in this study was also significantly impaired in MY-treated rats. In conclusion, MY impairs the estrous cycle and ovarian folliculogenesis in female rats by inhibiting the secretion of FSH and estradiol from the ovary, and inducing the oxidative stress in hypothalamic-pituitary-gonadal axis. © 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 2057-2067, 2016.
金莲橙黄（MY）是印度西孟加拉邦最常用的食用色素。在动物模型中，已经分别报道了MY对雄性生殖系统的毒性作用。迄今为止，尚未报道MY对雌性生殖功能可能产生的毒性作用。因此，本研究旨在探讨MY对雌性大鼠发情周期节律性和卵巢卵泡生成的影响。将大鼠暴露于250、500、750 mg/kg体重/天三种剂量的MY中，暴露持续时间为20天和30天。与对照大鼠相比，我们观察到在接受MY处理的大鼠中，发情周期的数量和持续时间有显著变化，同时发情周期各阶段阴道涂片细胞特征的细胞结构也有明显变化，且呈剂量和时间依赖性。我们还观察到接受MY处理的大鼠血清促卵泡激素（FSH）、促黄体生成素（LH）和雌二醇水平显著降低。此外，接受MY处理的大鼠脑组织中一些抗氧化酶的活性显著降低，而脂质过氧化标志物丙二醛（MDA）在接受MY处理的大鼠脑组织中的水平也显著升高。在本研究中，接受MY处理的大鼠的卵巢卵泡生成也受到显著损害。总之，MY通过抑制卵巢中FSH和雌二醇的分泌，并在下丘脑-垂体-性腺轴诱导氧化应激，损害雌性大鼠的发情周期和卵巢卵泡生成。© 2015威利期刊公司。《环境毒理学》31: 2057 - 2067, 2016。
