Xu Yingyin, Lu Yuxiao, Zhang Rui, Wang Hexiang, Liu Qinghong
State Key Laboratory for Agro-biotechnology, Department of Microbiology and Immunology, China Agricultural University. Beijing, People's Republic of China.
Department of Envioronmental and Chemical Engenineering, Tangshan College, People's Republic of China.
Acta Biochim Pol. 2016;63(2):273-9. doi: 10.18388/abp.2015_1091. Epub 2015 Oct 24.
A novel laccase was purified from the white rot fungus, Hohenbuehelia serotina, to investigate the applications of this laccase in the decoloration of various dyes. SDS-PAGE revealed a single band of this laccase corresponding to a molecular weight of approximately 57.8 kDa. The enzyme showed activity towards several substrates, the most sensitive of which was 2,2'-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS). The highest enzymatic activity using ABTS as a substrate was observed at pH 6.8 and 30°C. The enzyme activity was found to be significantly enhanced in the presence of Zn(2+) ions and inhibited by Fe(2+) ions. Moreover, SDS and β-mercaptoethanol were inhibitory, and inhibition by L-cysteine was observed while EDTA and DMSO had almost no inhibitory effect. The laccase could effectively decolorize seven different dyes within 30 minutes at 40°C.
从白腐真菌 Hohenbuehelia serotina 中纯化出一种新型漆酶，以研究该漆酶在各种染料脱色中的应用。SDS-PAGE 显示该漆酶有一条单一的条带，对应分子量约为 57.8 kDa。该酶对几种底物表现出活性，其中最敏感的底物是 2,2'-联氮双(3-乙基苯并噻唑啉-6-磺酸)（ABTS）。以 ABTS 为底物时，在 pH 6.8 和 30°C 下观察到最高酶活性。发现该酶活性在 Zn(2+)离子存在下显著增强，而受到 Fe(2+)离子抑制。此外，SDS 和 β-巯基乙醇具有抑制作用，观察到 L-半胱氨酸有抑制作用，而 EDTA 和 DMSO 几乎没有抑制作用。该漆酶在 40°C 下 30 分钟内可有效使七种不同染料脱色。
