基于铕（Ⅲ）荧光标记的羟基磷灰石纳米颗粒体外及体内定量检测方法
Quantitative Detection Method of Hydroxyapatite Nanoparticles Based on Eu(3+) Fluorescent Labeling in Vitro and in Vivo.
作者信息
Xie Yunfei, Perera Thalagalage Shalika Harshani, Li Fang, Han Yingchao, Yin Meizhen
机构信息
State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology , Wuhan 430070, P.R. China.
Biomedical Materials and Engineering Research Center of Hubei Province, Wuhan University of Technology , Wuhan 430070, P.R. China.
出版信息
ACS Appl Mater Interfaces. 2015 Nov 4;7(43):23819-23. doi: 10.1021/acsami.5b08767. Epub 2015 Oct 27.
One major challenge for application of hydroxyapatite nanoparticles (nHAP) in nanomedicine is the quantitative detection method. Herein, we exploited one quantitative detection method for nHAP based on the Eu(3+) fluorescent labeling via a simple chemical coprecipitation method. The trace amount of nHAP in cells and tissues can be quantitatively detected on the basis of the fluorescent quantitative determination of Eu(3+) ions in nHAP crystal lattice. The lowest concentration of Eu(3+) ions that can be quantitatively detected is 0.5 nM using DELFIA enhancement solution. This methodology can be broadly applicable for studying the tissue distribution and metabolization of nHAP in vivo.
羟基磷灰石纳米颗粒（nHAP）在纳米医学应用中的一个主要挑战是定量检测方法。在此，我们通过一种简单的化学共沉淀法，开发了一种基于铕（Eu(3+)）荧光标记的nHAP定量检测方法。基于对nHAP晶格中Eu(3+)离子的荧光定量测定，可对细胞和组织中的痕量nHAP进行定量检测。使用DELFIA增强溶液可定量检测的Eu(3+)离子的最低浓度为0.5 nM。该方法可广泛应用于研究nHAP在体内的组织分布和代谢。
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