Wojciechowicz Tatiana, Skrzypski Marek, Kołodziejski Paweł A, Szczepankiewicz Dawid, Pruszyńska-Oszmałek Ewa, Kaczmarek Przemysław, Strowski Mathias Z, Nowak Krzysztof W
Department of Animal Physiology and Biochemistry, Poznań University of Life Sciences, Poznań 60‑637, Poland.
Department of Hepatology and Gastroenterology and the Interdisciplinary Centre of Metabolism: Endocrinology, Diabetes and Metabolism, Charite‑University Medicine Berlin, Berlin D‑13353, Germany.
Mol Med Rep. 2015 Dec;12(6):8169-75. doi: 10.3892/mmr.2015.4470. Epub 2015 Oct 22.
Obestatin is a 23-amino acid peptide encoded by the ghrelin gene, which regulates food intake, body weight and insulin sensitivity. Obestatin influences glucose and lipid metabolism in mature adipocytes in rodents. However, the role of this peptide in rat preadipocytes remains to be fully understood. The current study characterized the effects of obestatin on lipid accumulation, preadipocyte differentiation, lipolysis and leptin secretion in rat primary preadipocytes. Obestatin enhanced lipid accumulation in rat preadipocytes and increased the expression of surrogate markers of preadipocyte differentiation. At the early stage of differentiation, obestatin suppressed lipolysis. By contrast, lipolysis was stimulated at the late stage of adipogenesis. Furthermore, obestatin stimulated the release of leptin, a key satiety hormone. Overall, the results indicated that obestatin promotes preadipocyte differentiation. Obestatin increased leptin release in preadipocytes, while the modulation of lipolysis appears to depend upon the stage of differentiation.
肥胖抑制素是一种由胃饥饿素基因编码的含23个氨基酸的肽，它调节食物摄入、体重和胰岛素敏感性。肥胖抑制素影响啮齿动物成熟脂肪细胞中的葡萄糖和脂质代谢。然而，这种肽在大鼠前脂肪细胞中的作用仍有待充分了解。当前的研究描述了肥胖抑制素对大鼠原代前脂肪细胞中脂质积累、前脂肪细胞分化、脂肪分解和瘦素分泌的影响。肥胖抑制素增强了大鼠前脂肪细胞中的脂质积累，并增加了前脂肪细胞分化替代标志物的表达。在分化早期，肥胖抑制素抑制脂肪分解。相比之下，在脂肪生成后期脂肪分解受到刺激。此外，肥胖抑制素刺激了关键饱腹感激素瘦素的释放。总体而言，结果表明肥胖抑制素促进前脂肪细胞分化。肥胖抑制素增加了前脂肪细胞中瘦素的释放，而脂肪分解的调节似乎取决于分化阶段。
