Ashiuchi Makoto, Hakumai Yuichi, Shibatani Shigeo, Hakuba Hirofumi, Oka Nogiho, Kobayashi Hisato, Yoneda Keizo
Graduate School of Integrated Arts and Sciences, Kochi University, Kochi 783-8502, Japan.
Faculty of Agriculture, Kochi University, Nankoku, Kochi 783-8502, Japan.
Int J Mol Sci. 2015 Oct 15;16(10):24588-99. doi: 10.3390/ijms161024588.
Poly-γ-glutamate (PGA) possesses a nylon-like backbone and polyacrylate-like carboxyl groups, and shows an extraordinary solubility in water. In this study, the effective synthesis and structural analysis of some water-insoluble PGA ion-complexes (PGAICs) using cationic surfactants, hexadecylpyridinium (HDP), dodecylpyridinium, benzalkonium and benzetonium, were examined. We demonstrated their spontaneous coating performance to the surfaces of different materials (i.e., plastics, metals, and ceramics) as potent anti-staphylococcal and anti-Candida agents. The tests against Staphylococcus aureus revealed that, regardless of a variety of materials, PGAICs maintained surface antimicrobial activity, even after the water-soaking treatment, whereas those against Candida albicans indicated that, among PGAICs, PGA/HDP complex is most useful as an anti-fungal agent because of its coating stability. Moreover, the log reduction values against Influenza A and B viruses of PGA/HDP-coated surfaces were estimated to be 5.4 and 3.2, respectively, suggesting that it can be dramatically suppressed the infection of influenza. This is to our knowledge the first observation of PGA-based antiviral coatings.
聚γ-谷氨酸（PGA）具有类似尼龙的主链和类似聚丙烯酸酯的羧基，在水中表现出非凡的溶解性。在本研究中，考察了使用阳离子表面活性剂十六烷基吡啶鎓（HDP）、十二烷基吡啶鎓、苯扎氯铵和苄索氯铵有效合成及结构分析一些水不溶性PGA离子复合物（PGAICs）的情况。我们证明了它们作为强效抗葡萄球菌和抗念珠菌剂对不同材料（即塑料、金属和陶瓷）表面的自发涂层性能。针对金黄色葡萄球菌的测试表明，无论材料种类如何，PGAICs即使在水浸处理后仍能保持表面抗菌活性，而针对白色念珠菌的测试表明，在PGAICs中，PGA/HDP复合物因其涂层稳定性而作为抗真菌剂最为有用。此外，PGA/HDP涂层表面对甲型和乙型流感病毒的对数减少值分别估计为5.4和3.2，表明它可以显著抑制流感感染。据我们所知，这是首次观察到基于PGA的抗病毒涂层。
