Anraku Makoto, Tabuchi Ryo, Ifuku Shinsuke, Ishiguro Takako, Iohara Daisuke, Hirayama Fumitoshi
Faculty of Pharmaceutical Sciences, Sojo University, 4-22-1 Ikeda, Nishi-Ku, Kumamoto 860-0082, Japan.
DDS Research Institute, Sojo University, 4-22-1 Ikeda, Nishi-Ku, Kumamoto 860-0082, Japan.
Int J Mol Sci. 2015 Oct 16;16(10):24707-17. doi: 10.3390/ijms161024707.
In this study, we examined a possible use of a surface-deacetylated chitin nano-fiber (SDCH-NF) and hyaluronic acid (HA) interpolymer complex (IPC) tablet as a potential antioxidative compound in extended-release matrix tablets. The antioxidant properties of untreated chitin (UCH), SDCH-NF, and HA were examined using N-centered radicals derived from 1,1'-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). SDCH-NF and HA had acceptable scavenging abilities and were relatively efficient radical scavengers, but UCH was much less effective. The results suggest that SDCH-NF and HA could serve as scavengers of compounds related to the development of oxidative stress. An SDCH-NF/HA IPC tablet was prepared and evaluated as an extended-release tablet matrix using famotidine (FMT) as a model drug. The release of FMT from the IPC tablet (DCF-NF:HA=1:1) was slower than that from a SDCH-NF only tablet. Turbidity measurements and X-ray diffraction (XRD) data also indicated that the optimum complexation ratio for IPC between SDCH-NF/HA is 1/1, resulting in a good relationship between turbidity or XRD of the complex and the release ratio of FMT. These results suggest that an SDCH-NF/HA tablet has the potential for use in an extended-release IPC tablet with a high antioxidant activity.
在本研究中，我们考察了表面脱乙酰化几丁质纳米纤维（SDCH-NF）与透明质酸（HA）互聚物复合物（IPC）片剂作为缓释基质片剂中潜在抗氧化化合物的可能用途。使用由1,1'-二苯基-2-苦基肼（DPPH）和2,2'-偶氮二异丁基脒二盐酸盐（ABTS）衍生的含氮自由基，检测了未处理几丁质（UCH）、SDCH-NF和HA的抗氧化性能。SDCH-NF和HA具有可接受的清除能力，是相对有效的自由基清除剂，但UCH的效果要差得多。结果表明，SDCH-NF和HA可作为与氧化应激发展相关化合物的清除剂。制备了SDCH-NF/HA IPC片剂，并以法莫替丁（FMT）为模型药物，作为缓释片剂基质进行评价。FMT从IPC片剂（DCF-NF:HA = 1:1）中的释放比从仅含SDCH-NF的片剂中释放得慢。浊度测量和X射线衍射（XRD）数据还表明，SDCH-NF/HA之间IPC的最佳络合比为1/1，这导致络合物的浊度或XRD与FMT的释放率之间具有良好的关系。这些结果表明，SDCH-NF/HA片剂有潜力用于具有高抗氧化活性的缓释IPC片剂。
