Laboratory of Toxicology, Ghent University , Ottergemsesteenweg 460, B-9000 Gent, Belgium.
Laboratory of Functional Plant Biology, Department of Physiology, Ghent University , K.L. Ledeganckstraat 35, B-9000 Gent, Belgium.
J Agric Food Chem. 2015 Nov 18;63(45):10089-95. doi: 10.1021/acs.jafc.5b04075. Epub 2015 Nov 9.
A method for the quantitative determination of folates in rodent diets is very important for correct interpretation of folate intake during feeding trials, given the possible discrepancy between the actual folate concentration in the diet and that mentioned on the product sheet. Liquid chromatography tandem-mass spectrometry is the method of choice to differentiate and quantify the individual folate species present. This discrepancy may be accounted for by, e.g., inaccurate folic acid supplementation and/or the presence of endogenous reduced and substituted folates. We developed a method, validated based on FDA guidelines, that allows the measurement of added and endogenous folates by quantitative determination of 5 folate monoglutamates with linear ranges from 8 μg to 2 mg/kg feed. This information, combined with feed intake data, allows insight into the actual folate intake in animal feeding studies. The relevance of this method was illustrated by the analysis of several feed samples of varying composition, by the investigation of the effect of casein incorporation, and by evaluating the variability of the folate content between pellets and production batches.
一种用于定量测定啮齿动物饲料中叶酸的方法非常重要，因为在喂养试验中，饲料中的实际叶酸浓度与产品标签上所提到的叶酸浓度之间可能存在差异。液相色谱串联质谱法是区分和定量测定存在的各种叶酸的首选方法。这种差异可能是由于叶酸补充不准确和/或存在内源性还原和取代的叶酸。我们开发了一种方法，该方法基于 FDA 指南进行了验证，可通过定量测定 5 种叶酸单谷氨酸盐来测量添加的和内源性的叶酸，线性范围为 8μg 至 2mg/kg 饲料。结合饲料摄入量数据，该信息可深入了解动物喂养研究中的实际叶酸摄入量。该方法的相关性通过对不同组成的几种饲料样品的分析、对酪蛋白掺入的影响的研究以及评估颗粒和生产批次之间叶酸含量的变异性得到了说明。
