在红色荧光蛋白（RFP）转基因裸鼠体内原位肝肿瘤对癌症相关成纤维细胞和血管的招募情况。

Recruitment of Cancer-Associated Fibroblasts and Blood Vessels by Orthotopic Liver Tumors Imaged in Red Fluorescent Protein (RFP) Transgenic Nude Mice.

作者信息

Suetsugu Atsushi, Hiroshima Yukihiko, Matsumoto Takuro, Hasagawa Kosuke, Nakamura Miki, Shimizu Masahito, Saji Shigetoyo, Moriwaki Hisataka, Bouvet Michael, Hoffman Robert M

机构信息

Gifu University Graduate School of Medicine, Gifu, Japan AntiCancer, Inc. San Diego, CA, U.S.A Department of Surgery, University of California, San Diego, CA, U.S.A.

AntiCancer, Inc. San Diego, CA, U.S.A Department of Surgery, University of California, San Diego, CA, U.S.A.

出版信息

Anticancer Res. 2015 Nov;35(11):5821-5.

PMID:26504003

Abstract

BACKGROUND/AIM: The tumor microenvironment (TME) is critical for tumor growth and progression. We report here an imageable model of the TME of orthotopic liver cancer.

MATERIALS AND METHODS

The transgenic red fluorescent protein (RFP)-expressing nude mouse was used as the host. The RFP nude mouse expresses RFP in all organs. Non-colored Huh-7 human hepatoma cells were injected in the spleen of RFP nude mice to establish an orthotopic liver cancer model. TME formation resulting from the orthotopic liver tumor was observed using the Olympus OV100 small animal fluorescence imaging system.

RESULTS

Non-colored liver cancer cells formed tumor colonies in the liver 28 days after cell transplantation to the spleen. RFP-expressing host cells and blood vessels were recruited by the liver tumors as visualized by fluorescence imaging. A desmin- and sirus-red-positive area increased around and within the liver tumor over time.

CONCLUSION

These results indicate cancer-associated fibroblasts (CAFs) were recruited by the liver tumors suggesting that CAFs, along with the angiogenic tumor blood vessels, were necessary for liver-tumor growth and could serve as visible therapeutic targets.

摘要

背景/目的：肿瘤微环境（TME）对肿瘤的生长和进展至关重要。我们在此报告一种原位肝癌TME的可成像模型。