Department of Surgery, University of California San Diego, San Diego, CA, U.S.A.
AntiCancer, Inc., San Diego, CA, U.S.A.
In Vivo. 2020 Nov-Dec;34(6):3153-3157. doi: 10.21873/invivo.12149.
BACKGROUND/AIM: Surgical orthotopic implantation (SOI) is used to establish patient-derived orthotopic xenograft (PDOX) and other orthotopic mouse models. Orthotopic liver models can be challenging, as the liver parenchyma is prone to bleeding. The present report describes a sutureless method to implant tumors in the liver that reduces bleeding and procedural time.
Human HCC cell-line (Huh-7-GFP) and CM2, a patient-derived colon-cancer liver metastasis, were used for sutureless SOI of tumor fragments in the liver of nude mice. A small cavity was formed on the liver surface. A solitary tumor fragment was implanted in the cavity without suturing to create hemostasis.
Six weeks after sutureless SOI, the tumor volume of Huh-7-GFP (n=5) was 584.41±147.64 mm and the tumor volume of CM2 (n=5) was 1336.54±1038.20 mm The engraftment rate was 100%.
This novel method for establishing orthotopic liver-implantation mouse models is suitable for studies of liver cancer and liver metastases due to its simple procedure and potential high engraftment rate.
背景/目的：外科原位种植（SOI）用于建立患者来源的原位异种移植（PDOX）和其他原位小鼠模型。由于肝脏实质容易出血，因此建立原位肝脏模型具有挑战性。本报告描述了一种减少出血和手术时间的无缝线肝脏肿瘤种植方法。
使用人肝癌细胞系（Huh-7-GFP）和 CM2（一种患者来源的结肠癌肝转移）进行无缝线 SOI，将肿瘤碎片原位种植到裸鼠肝脏中。在肝表面形成一个小腔。将单个肿瘤碎片植入腔中，无需缝合以止血。
无缝线 SOI 后 6 周，Huh-7-GFP（n=5）的肿瘤体积为 584.41±147.64mm，CM2（n=5）的肿瘤体积为 1336.54±1038.20mm，种植率为 100%。
这种建立原位肝脏植入小鼠模型的新方法由于其简单的程序和潜在的高种植率，适用于肝癌和肝转移的研究。
