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用于检测人体弓蛔虫病的免疫诊断方法。

Immunodiagnostic approaches for the detection of human toxocarosis.

作者信息

Boldiš Vojtech, Ondriska František, Špitalská Eva, Reiterová Katarína

机构信息

HPL (Ltd) Medical Laboratories, Istrijská 20, 84107 Bratislava, Slovakia.

HPL (Ltd) Medical Laboratories, Istrijská 20, 84107 Bratislava, Slovakia.

出版信息

Exp Parasitol. 2015 Dec;159:252-8. doi: 10.1016/j.exppara.2015.10.006. Epub 2015 Oct 23.

Abstract

Human toxocarosis is an important zoonosis caused by larvae of Toxocara canis/cati. The objective was to evaluate the role of IgG anti-Toxocara antibody detection and the specific IgG avidity in diagnostics of human toxocarosis. Anti-Toxocara IgG antibodies and IgG avidity were evaluated by excretory-secretory (ES)-enzyme-linked immunosorbent assay (ELISA). The IgG anti-Toxocara seroprevalence in people (n = 7678) from western Slovakia was 15.3% and found to be highest in the oldest age groups. The presence of low- IgG avidity in 179 suspected patients for toxocarosis was evaluated in relation to sex, age, IgG antibody levels, eosinophilia, increased total IgE, domicile, geophagia, dog/cat ownership, anamnesis. Low- IgG avidity index was found in 30.7% of the patients. The low- IgG avidity in eosinophilic group (42.1%) was significantly higher than in non-eosinophilic group (22.0%; P = 0.043). Substantially higher eosinophilia was detected in children (under 10 years old; 55.6%) than in adults (aged ≥ 41 years; 17.6%; P = 0.009). Significant difference between seroprevalence of total IgE in patients coming from towns (48.8%) and patients from villages (21.3%) was established (P = 0.007). Mild negative correlation (r = -0.477, P = 0.043) was observed between the amounts of eosinophils and the values of IgG avidity. The sensitivity and specificity of IgG avidity assay were 43.8% and 83.3%, respectively. Our results suggest that besides anti-Toxocara IgG, measurement of IgG avidity may be useful for the determination of acute toxocarosis. Moreover, these tests should be accompanied by other immunological markers and determinants of examined patients such as eosinophilia, increased total IgE and age.

摘要

人体弓首蛔虫病是由犬弓首蛔虫/猫弓首蛔虫幼虫引起的一种重要的人畜共患病。目的是评估抗弓首蛔虫IgG抗体检测及特异性IgG亲和力在人体弓首蛔虫病诊断中的作用。通过排泄分泌(ES)-酶联免疫吸附测定(ELISA)评估抗弓首蛔虫IgG抗体和IgG亲和力。来自斯洛伐克西部的人群(n = 7678)中抗弓首蛔虫IgG血清阳性率为15.3%,且在年龄最大的组中最高。对179例疑似弓首蛔虫病患者的低IgG亲和力情况与性别、年龄、IgG抗体水平、嗜酸性粒细胞增多、总IgE升高、居住地、食土癖、养狗/猫情况、既往史进行了评估。30.7%的患者检测到低IgG亲和力指数。嗜酸性粒细胞组的低IgG亲和力(42.1%)显著高于非嗜酸性粒细胞组(22.0%;P = 0.043)。儿童(10岁以下;55.6%)的嗜酸性粒细胞增多情况明显高于成年人(年龄≥41岁;17.6%;P = 0.009)。来自城镇患者(48.8%)和来自乡村患者(21.3%)的总IgE血清阳性率存在显著差异(P = 0.007)。嗜酸性粒细胞数量与IgG亲和力值之间观察到轻度负相关(r = -0.477,P = 0.043)。IgG亲和力测定的敏感性和特异性分别为43.8%和83.3%。我们的结果表明,除了抗弓首蛔虫IgG外,IgG亲和力的检测可能有助于急性弓首蛔虫病的诊断。此外,这些检测应结合其他免疫标志物以及受检患者的决定因素,如嗜酸性粒细胞增多、总IgE升高和年龄。

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