Uhlíková M, Hübner J, Kolárová L, Polácková M
Postgraduate Medical School, Prague, Czech Republic.
Cent Eur J Public Health. 1996 Dec;4(4):242-5.
The aim of the study was to characterize the antiparasite humoral response in patients with the syndrome of visceral larval toxocarosis. Specific IgG, specific IgE and total IgE immunoglobulins against Toxocara canis excretory/secretory antigens (TES) were detected by using ELISA technique. Antibody response was studied in complete sera as well as in immunoglobulin fractions (IgG and IgE), isolation of which was performed on Protein A Sepharose. It was observed that removal of IgG from the serum samples resulted mostly in increasing levels of anti-Toxocara IgE antibodies what agrees with the theory of the blocking effect of IgG in the immune response. The results demonstrated a little correlation between slgG and slgE in the sera of symptomatic patients, examined in ELISA reaction.
本研究的目的是对内脏幼虫弓蛔虫病综合征患者的抗寄生虫体液反应进行特征描述。采用酶联免疫吸附测定（ELISA）技术检测针对犬弓蛔虫排泄/分泌抗原（TES）的特异性IgG、特异性IgE和总IgE免疫球蛋白。在完整血清以及免疫球蛋白组分（IgG和IgE）中研究抗体反应，后者通过蛋白A琼脂糖凝胶进行分离。观察到从血清样本中去除IgG大多会导致抗弓蛔虫IgE抗体水平升高，这与IgG在免疫反应中的阻断作用理论相符。结果表明，在ELISA反应中检测的有症状患者血清中，分泌型IgG（slgG）和分泌型IgE（slgE）之间存在微弱相关性。
