去甲肾上腺素对大鼠棕色脂肪细胞中三酰甘油合成的影响。
Effect of noradrenaline on triacylglycerol synthesis in rat brown adipocytes.
作者信息
Baht H S, Saggerson E D
机构信息
Department of Biochemistry, University College London, U.K.
出版信息
Biochem J. 1989 Mar 1;258(2):369-73. doi: 10.1042/bj2580369.
Abstract
Incubation of rat brown adipocytes with noradrenaline in the presence of insulin and palmitate caused a decrease in the rate of triacylglycerol synthesis as measured by [U-14C]glucose incorporation into acylglycerol glycerol. Concomitantly, the ratio of [1-14C]palmitate oxidized to CO2 to that esterified was increased. This alteration in the rate of triacylglycerol synthesis by noradrenaline was not observed when fatty acid oxidation was inhibited by etomoxir. Noradrenaline did not cause any acute inactivation of enzymes of the triacylglycerol-synthesis pathway. It is suggested that the decrease in triacylglycerol synthesis seen with noradrenaline is secondary to activation of fatty acid oxidation.
摘要
在胰岛素和棕榈酸存在的情况下，用去甲肾上腺素孵育大鼠棕色脂肪细胞，会导致三酰甘油合成速率降低，这是通过[U-14C]葡萄糖掺入酰基甘油甘油来测量的。与此同时，[1-14C]棕榈酸氧化为二氧化碳与酯化的比例增加。当用依托莫昔抑制脂肪酸氧化时，未观察到去甲肾上腺素引起的三酰甘油合成速率的这种改变。去甲肾上腺素不会导致三酰甘油合成途径的酶发生任何急性失活。有人提出，去甲肾上腺素导致的三酰甘油合成减少是脂肪酸氧化激活的继发结果。
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