Petrochenko Peter E, Kumar Girish, Fu Wujun, Zhang Qin, Zheng Jiwen, Liang Chengdu, Goering Peter L, Narayan Roger J
J Biomed Nanotechnol. 2015 Dec;11(12):2275-85. doi: 10.1166/jbn.2015.2169.
The surface topographies of nanoporous anodic aluminum oxide (AAO) and titanium dioxide (TiO2) membranes have been shown to modulate cell response in orthopedic and skin wound repair applications. In this study, we: (1) demonstrate an improved atomic layer deposition (ALD) method for coating the porous structures of 20, 100, and 200 nm pore diameter AAO with nanometer-thick layers of TiO2 and (2) evaluate the effects of uncoated AAO and TiO2-coated AAO on cellular responses. The TiO2 coatings were deposited on the AAO membranes without compromising the openings of the nanoscale pores. The 20 nm TiO2-coated membranes showed the highest amount of initial protein adsorption via the micro bicinchoninic acid (micro-BCA) assay; all of the TiO2-coated membranes showed slightly higher protein adsorption than the uncoated control materials. Cell viability, proliferation, and inflammatory responses on the TiO2-coated AAO membranes showed no adverse outcomes. For all of the tested surfaces, normal increases in proliferation (DNA content) of L929 fibroblasts were observed over from 4 hours to 72 hours. No increases in TNF-alpha production were seen in RAW 264.7 macrophages grown on TiO2-coated AAO membranes compared to uncoated AAO membranes and tissue culture polystyrene (TCPS) surfaces. Both uncoated AAO membranes and TiO2-coated AAO membranes showed no significant effects on cell growth and inflammatory responses. The results suggest that TiO2-coated AAO may serve as a reasonable prototype material for the development of nanostructured wound repair devices and orthopedic implants.
纳米多孔阳极氧化铝（AAO）和二氧化钛（TiO₂）膜的表面形貌已被证明可调节骨科和皮肤伤口修复应用中的细胞反应。在本研究中，我们：（1）展示了一种改进的原子层沉积（ALD）方法，用于用纳米厚的TiO₂层涂覆孔径为20、100和200 nm的AAO多孔结构，以及（2）评估未涂覆的AAO和TiO₂涂覆的AAO对细胞反应的影响。TiO₂涂层沉积在AAO膜上，而不影响纳米级孔隙的开口。通过微量双辛可宁酸（微量BCA）测定，20 nm TiO₂涂层膜显示出最高的初始蛋白质吸附量；所有TiO₂涂层膜的蛋白质吸附量均略高于未涂覆的对照材料。TiO₂涂层的AAO膜上的细胞活力、增殖和炎症反应均未显示出不良结果。对于所有测试表面，在4小时至72小时内观察到L929成纤维细胞增殖（DNA含量）正常增加。与未涂覆的AAO膜和组织培养聚苯乙烯（TCPS）表面相比，在TiO₂涂层的AAO膜上生长的RAW 264.7巨噬细胞中TNF-α的产生没有增加。未涂覆的AAO膜和TiO₂涂层的AAO膜对细胞生长和炎症反应均无显著影响。结果表明，TiO₂涂层的AAO可作为开发纳米结构伤口修复装置和骨科植入物的合理原型材料。
