Fagone Paolo, Nicoletti Ferdinando, Vecchio Giada Maria, Parenti Rosalba, Magro Gaetano
Department of Biomedical Sciences, School of Medicine, University of Catania, Catania, Italy.
Department of Medical and Surgical Sciences and Advanced Technologies, G.F. Ingrassia, Azienda Ospedaliero-Universitaria "Policlinico-Vittorio Emanuele", Anatomic Pathology Section, School of Medicine, University of Catania, Catania, Italy.
Acta Histochem. 2015 Oct;117(8):820-3. doi: 10.1016/j.acthis.2015.10.002. Epub 2015 Oct 25.
Neuroblastoma is the most common extracranial solid childhood tumor, which is believed to originate from primitive neuroblasts giving rise to the sympathetic nervous system. It was previously shown that cyclin D1 (CCDN1) in pediatric neuroblastic tumors (neuroblastoma, ganglioneuroblastoma, and ganglioneuroma) recapitulates its expression during the development of peripheral sympathetic nervous system (PSNS). In the present study, we performed a microarray analysis in order to evaluate the expression of cyclin D1 in neuroblastoma as compared to ganglioneuroma and ganglioneuroblastoma. We first confirmed that comparable levels of cyclin D1 are present in neuroblastoma and fetal neuroblasts. In addition, we observed that neuroblastoma is associated to significantly higher levels of cyclin D1 as compared to both ganglioneuroma and ganglioneuroblastoma. No differences are instead observable between ganglioneuroblastoma and ganglioneuroma. Finally, bioinformatic analysis of cyclin D1-functionally related genes, identified cyclin D2 as an additional marker/etiopathogenic factor in the development of neuroblastoma.
神经母细胞瘤是儿童最常见的颅外实体瘤，被认为起源于产生交感神经系统的原始神经母细胞。先前的研究表明，小儿神经母细胞瘤（神经母细胞瘤、神经节神经母细胞瘤和神经节神经瘤）中的细胞周期蛋白D1（CCDN1）在外周交感神经系统（PSNS）发育过程中重现其表达。在本研究中，我们进行了微阵列分析，以评估与神经节神经瘤和神经节神经母细胞瘤相比，神经母细胞瘤中细胞周期蛋白D1的表达。我们首先证实神经母细胞瘤和胎儿神经母细胞中存在相当水平的细胞周期蛋白D1。此外，我们观察到，与神经节神经瘤和神经节神经母细胞瘤相比，神经母细胞瘤中细胞周期蛋白D1的水平显著更高。相反，神经节神经母细胞瘤和神经节神经瘤之间没有观察到差异。最后，对细胞周期蛋白D1功能相关基因的生物信息学分析确定细胞周期蛋白D2是神经母细胞瘤发生发展中的另一个标志物/致病因素。
